William S. Reznikoff

Photo of William S. Reznikoff
Emeritus Professor 2007-Present
Professor 1970-2007
Evelyn Mercer Professor
B.A., Williams College
Ph.D., The Johns Hopkins University
Email: reznikoff@biochem.wisc.edu

Curriculum Vitae

Education

1963 - B. A. Biology, Williams College, Williamstown, MA

1961-1962 Special Student, University of Ghana, Accra, Ghana

1963-1967 Predoctoral Fellow, Biology, The Johns Hopkins University, Baltimore, MD

1964 Research Assistant, Biology, Oak Ridge National Lab. Oak Ridge, TN

1967 - Ph. D. Biology, The Johns Hopkins University, Baltimore, MD

1970 - Postdoctoral Fellow, Harvard Medical School, Boston, MA
Research activities: Bacterial molecular genetics: Analysis of lac and trp operon DNA regulatory sites

Professional appointments

1970-1975 Assistant Professor, Department of Biochemistry, University of Wisconsin-Madison

1975-1978 Associate Professor, Department of Biochemistry, University of Wisconsin-Madison

1978-1985 Professor, Department of Biochemistry, University of Wisconsin-Madison

1985-2007 Evelyn Mercer Professor of Department of Biochemistry and Molecular Biology, University of Wisconsin-Madison

07/1986-06/1991 Chairman, Department of Biochemistry, University of Wisconsin-Madison

01/2004-08/2004 Visiting Scientist Marine Biological, Bay Paul Center for Comparative Molecular Biology and Evolution, Laboratory, Woods Hole, MA

06/2005-2007 Adjunct Scientist, Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA

2007-present Professor Emeritus, Department of Biochemistry, University of Wisconsin-Madison

2007-present Senior Research Scientist Biology and Evolution, Bay Paul Center for Comparative Molecular , Marine Biological Laboratory, Woods Hole, MA

Distinctions and awards

1963 Phi Beta Kappa, Williams College, Williamstown, MA

1963-1967 NIH predoctoral fellow, Johns Hopkins University, Baltimore, MD

1968-1969 NIH postdoctoral fellow, Harvard Medical School, Boston, MA

1970 Medical Research Foundation of Boston postdoctoral fellow, Harvard Medical School, Boston, MA

1972-1977 NIH Career Development Award, University of Wisconsin-Madison

1974-1978 Harry and Evelyn Steenbock Career Development award, University of Wisconsin-Madison

1984-1985 Faculty Star Award, University of Wisconsin-Madison

1985-present Evelyn Mercer Professor of Biochemistry and Molecular Biology, University of Wisconsin-Madison

1997 Vilas Associate Award, University of Wisconsin-Madison

2002 Spitze Land Grant Faculty Award, University of Wisconsin-Madison

2006 Arthur J. Maurer Extra Mile Award, University of Wisconsin-Madison

External awards

1981 Pasteur Award, Illinois Society for Microbiology

1986 George A. Miller Visiting Professor of Microbiology, Univ. of Illinois

1989 Oliver H. Smith Memorial Lecturer, Marquette University

2001 American Society of Microbiology Division H Lecturer

2001 Elected Fellow, American Academy of Arts and Sciences

Other activities

Consulting and Study Section Service

1976-1979 NSF, Advisory Subcommittee (study section) for Genetic Biology

1980-1983 NIGMS, Cellular Basis of Disease Review Committee (study section for training grant reviews)

1983-1984 NIGMS, Chair, Cellular Basis of Disease Review Committee (study section for training grant reviews)

1980-1985 Consultant, Biogen S. A., Geneva, Switzerland

1981-1996 Member, Board of Directors, Promega Corp.

1987 Governor's Council on Biotechnology

1996-2005 Member, Scientific Advisory Board, Promega Corp.

Editorial Board Service

1971-1985 Journal of Bacteriology

1989-1991 Journal of Biological Chemistry

1993-1995 Journal of Biological Chemistry

1997-present Biochemical and Biophysical Research Communications

2002-present Critical Reviews in Biochemistry and Molecular Biology

Meeting Organizing

1976 Cold Spring Harbor Meeting on "The Operon", co-organizer

1986 Steenbock Symposium - RNA Polymerase and the Regulation of Transcription, University of Wisconsin, co-organizer

1996, 97 & 98 E. coli Genome Meetings - University of Wisconsin, co-organizer

1998 Molecular Genetics of Bacteria and Phages - Cold Spring Harbor, co-organizer

1999 E. coli Genome Meeting - Lake Arrowhead, co-organizer

2004 Steenbock Symposium: Gene Expression, Transposition, Genomics and Other Life Sciences, University of Wisconsin, co-organizer

2006-2007 Mobile Genetic Element Cluster, Marine Biological Laboratory, co-organizer

2007 Northeast Mobile Genetic Element Meeting, Marine Biological Laboratory, co-organizer

Major University Duties:

1981-1997 Cellular and Molecular Biology (Molecular Biosciences) Training Grant, director

1986-1991 Department of Biochemistry, chair

1995-1996 University Committee, chair

1995-1996 University Committee, co-chair

Laboratory Training

I have trained 43 graduate students (39 Ph. D. and 4 masters), 13 postdoctoral fellows and 6 visiting scholars.

Publications

1. Reznikoff, W. S. and C. A. Thomas, Jr. (1969).  The anatomy of the SP50 bacteriophage DNA molecule. Virology 37, 309. [PDF]

2. Silverstone, A. E., B. Magansanik, W. S. Reznikoff, J. H. Miller, and J. R. Beckwith. (1969).  Catabolite sensitive site of the lac operon. Nature 221, 1012. [PDF]

3. Reznikoff, W. S., J. H. Miller, J. G. Scaife, and J. R. Beckwith. (1969).  A mechanism for repressor action. J. Mol. Biol. 43, 201. [PDF]

4. Reznikoff, W. S. and J. R. Beckwith. (1969).  Genetic evidence that the operator locus is distinct from the Z gene in the lac operon of Escherichia coli. J. Mol. Biol. 43, 215. [PDF]

5. Miller, J. H., W. S. Reznikoff, A. E. Silverstone, D. Ippen, E. R. Signer, and J. R. Beckwith. (1970). Transposition of the lac region of E. coli. V. Fusions of the lac and trp regions of the E. coli chromosome. J. Bacteriol. 104, 1273. [PDF]

6. Michels, C. A. and W. S. Reznikoff. (1971). The gradient of polarity of Z gene nonsense mutations in trp-lac fusion strains. J. Mol. Biol. 55, 119. [PDF]

7. Eron, L., D. Morse, W. Reznikoff, and J. Beckwith. (1971). Fusions of the lac and trp regions of Escherichia coli: Covalently fused messenger RNA. J. Mol. Biol. 60, 203. [PDF]

8. Reznikoff, W. S. and K. P. Thornton. (1972). Isolation of tryptophan regulatory mutants in Escherichia coli using trp-lac fusion strain. J. Bacteriol. 109, 526. [PDF]

9. Reznikoff, W. S. (1972). The operon revisited. Annu. Rev. Genet 6, 132. [PDF]

10. Barnes, W. M., R. B. Siegel, and W. S. Reznikoff. (1974). The construction of lambda transducing phages containing deletions defining regulatory elements of the lac and trp operons in E. coli. Mol. Gen. Genet. 129, 201-215.

11. Reznikoff, W. S., C. A. Michels, T. G. Cooper, A. E. Silverstone, and B. Magasanik. (1974). Inhibition of lacZ gene translation initiation in trp-lac fusion strains. J. Bacteriol. 117, 1231. [PDF]

12. Reznikoff, W. S., R. B. Winter, and C. K. Hurley. (1974). The location of the repressor binding sites in the lac operon. Proc. Natl. Acad. Sci. USA 71, 2314. [PDF]

13. Dickson, R. C., J. Abelson, W. M. Barnes, and W. S. Reznikoff. (1975). Genetic regulation: The lac control region. Science 187, 27. [PDF]

14. Mitchell, D., W. Reznikoff, and J. Beckwith. (1975). Genetic fusions defining trp and lac operon regulatory elements. J. Mol. Biol. 93, 331. [PDF]

15. Vinopal, R. T., J. D. Hillman, H. Schulman, W. S. Reznikoff, and D. G, Fraenkel. (1975). New phosphoglucose isomerase mutants of Escherichia coli. J. Bacteriol. 122, 1172. [PDF]

16. Barnes, W. M., W. S. Reznikoff, F. R. Blattner, R. C. Dickson, and J. Abelson. (1975). The isolation of RNA homologous to the genetic control elements of the lactose operon. J. Biol. Chem. 250, 8184. [PDF]

17. Aline, R. F., Jr. and W. S. Reznikoff. (1975). Bacteriophage Mul induced permeability mutants in Escherichia coli K-12. J. Bacteriol. 124, 578. [PDF]

18. Mitchell, D. H., W. S. Reznikoff, and J. Beckwith. (1976). Genetic fusions that help define a transcription termination region in Escherichia coli. J. Mol. Biol. 101, 441. [PDF]

19. Reznikoff, W. S. (1976). Formation of the RNA polymerase-lac promoter open complex. In RNA Polymerase, Cold Spring Harbor Laboratory, pp. 441-454.

20. Jones, B. B. and Reznikoff, W. S. (1976). Isolation of specialized transducing bacteriophages carrying deletions of the regulatory region of the Escherichia coli K-12 tryptophan operon. J. Bacteriol. 128, 283. [PDF]

21. Dickson, R. C., J. Abelson, P. Johnson, W. S. Reznikoff, and W. M. Barnes. (1977). Nucleotide sequence changes produced by mutations in the lac promoter of Escherichia coli. J. Mol. Biol. 111, 65. [PDF]

22. Jones, B. B., C. Hardy, S. Rothstein, R. D. Wells, and W. S. Reznikoff. (1977). RNA polymerase binding sites in lambda plac5 DNA. Proc. Natl. Acad. Sci. USA 74, 4914. [PDF]

23. Jones, B. B. and W. S. Reznikoff. (1977). Tryptophan-transducing bacteriophages: In vitro studies with restriction endonucleases HindII + III and Escherichia coli ribonucleic acid polymerase. J. Bacteriol. 132, 270. [PDF]

24. Jorgensen, R., D. Berg, and W. S. Reznikoff. (1978). Genetic organization in the transposable tetracycline resistance determinant Tnl0. In Microbiology 1978, (D. Schlessinger, ed. ), Am. Soc. for Microbiol., Washington, D. C.

25. Reznikoff, W. S. and J. N. Abelson. (1978). The lac promoter. In The Operon, (Miller & Reznikoff, eds. ), Cold Spring Harbor Laboratory, pp. 221-243.

26. Postle, K. and W. S. Reznikoff. (1978). HindII and HindIII restriction maps of the attphi80-tonB-trp region of the Escherichia coli genome, and location of the tonB gene. J. Bacteriol. 136, 1165 [PDF]

27. Maquat, L. E. and W. S. Reznikoff. (1978). In vitro analysis of the Escherichia coli RNA polymerase interaction with wild-type and mutant lactose promoters. J. Mol. Biol. 125, 467. [PDF]

28. Jorgensen, R. A., D. E. Berg, B. Allet, and W. S. Reznikoff. (1979). Restriction enzyme cleavage map of Tnl0, a transposon which encodes tetracycline resistance. J. Bacteriol. 137, 681. [PDF]

29. Gardner, J. F. and W. S. Reznikoff. (1978). Identification and restriction endonuclease mapping of the threonine operon regulatory region. J. Mol. Biol. 126, 241. [PDF]

30. Jorgensen, R. A. and W. S. Reznikoff. (1979). Organization of structural and regulatory genes that mediate tetracycline resistance in transposon Tnl0. J. Bacteriol. 138, 705. [PDF]

31. Postle, K. and W. S. Reznikoff. (1979). Identification of the Escherichia coli tonB gene product in minicells containing tonB hybrid plasmids. J. Mol. Biol. 131, 619. [PDF]

32. Hardies , S. C., R. K. Patient, R. D. Klein, F. Ho, W. S. Reznikoff, and R. Wells. D. (1979). Construction and mapping of the recombinant plasmids used for the preparation of DNA fragments containing the Escherichia coli lactose operator and promoter. J. Biol. Chem. 254, 5527. [PDF]

33. Jorgensen, R. A., S. J. Rothstein, and W. S. Reznikoff. (1979). A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance. Mol. Gen. Genet. 177, 65.

34. Rothstein, S. J., R. A. Jorgensen, K. Postle, and W. S. Reznikoff. (1980). The inverted repeats of Tn5 are functionally different. Cell 19, 795. [PDF]

35. Wartell, R. M. and W. S. Reznikoff. ( 1980). Cloning DNA restriction endonuclease fragments with protruding single stranded ends. Gene 9, 307. [PDF]

36. Maquat, L. E., K. Thorton, and W. S. Reznikoff. (1980). Lac promoter mutations located downstream from the transcription start site. J. Mol. Biol. 139, 537. [PDF]

37. Maquat, L. E. and W. S. Reznikoff. (1980). The lac promoter mutation pr 115 generates a new transcription initiation point. J. Mol. Biol. 139, 5 51. [PDF]

38. Rothstein, S. J., R. A. Jorgensen, J. C. -P. Yin, Z. Yong-di, R. C. Johnson, and W. S. Reznikoff, W. S. (1980). Genetic organization of Tn5. Cold Spring Harbor Symp. Quant. Biol. 45, 99-105.

39. Rothstein, S. J. and W. S, Reznikoff. (1981). The functional differences in the inverted repeats of Tn5 are caused by a single base pair nonhomology. Cell 23, 191-199. [PDF]

40. Munson, L. M. and W. S. Reznikoff. (1981). Abortive initiation and long RNA synthesis. Biochemistry 20, 2081-2085. [PDF]

41. Johnson, R. C. and W. S. Reznikoff. (1981). Localization of the Tn5 transposase promoter using the recycling reaction of RNA polymerase. Nucleic Acids Res. 9, 1873-1882. [PDF]

42. Wray, L. V., R. A. Jorgensen, and W. S. Reznikoff. (1981). Identification of the tetracycline resistance promoter and repressor in transposonTnl0. J. Bacteriol. 147, 297-304. [PDF]

43. Reznikoff, W. S., S. J. Rothstein, R. A. Jorgensen, R. C. Johnson, and J. C. -P. Yin. (1981). The structure of Tn5. In Molecular Biology, Pathogenicity, and Ecology of Bacterial Plasmids, (Levy, Clowes and Koenig, eds. ), Plenum Press, New York, pp. 371-380.

44. Reznikoff, W. S., L. E. Maquat, L. M. Munson, R. C. Johnson, and W. Mandecki. (1982). The lac promoter: Analysis of structural signals for transcription initiation and identification of a new sequence specific event. In Promoters: Structure and Function, (Rodriguez and Chamberlin, eds. ), Praeger, New York, pp. 80-95.

45. Mandecki, W. and W. S. Reznikoff. (1982). A lac promoter with a changed distance between -10 and -35 regions. Nucleic Acids Res. 10, 903-912. [PDF]

46. Hillen, W., G. Klock, I. Kaffenberger, L. V. Wray, and W. S. Reznikoff. (1982). Purification of the TET repressor and TET operator from the transposon Tnl0 and characterization of their interaction. J. Biol. Chem. 257, 6605-6613. [PDF]

47. Lynn, S. L., J. F. Gardner, and W. S. Reznikoff. (1982). Attenuation regulation in the threonine operon of Escherichia coli K-12: Molecular cloning and transcription of the controlling region. J. Bacteriol. 152, 363-371. [PDF]

48. Johnson, R. C., J. C. -P. Yin, and W. S. Reznikoff. (1982). The control of Tn5 transposition in Escherichia coli is mediated by protein from the right repeat. Cell 30, 873-882. [PDF]

49. Reznikoff, W S. (1982). Tn5 transposition and its regulation. Cell 31, 307-308.

50. Bertrand, K. P., K. Postle, L. V. Wray, Jr., and W. S. Reznikoff. (1983). Overlapping divergent promoters control expression of Tnl0 tetracycline resistance. Gene 23, 149-156. [PDF]

51. Johnson, R. C. and W. S. Reznikoff. (1983). DNA sequences at the ends of transposon Tn5 required for transposition. Nature 304, 280-282. [PDF]

52. Wray, L. V., Jr. and Reznikoff, W. S. (1983). Identification of the repressor binding sites controlling the expression of tetracycline resistance encoded by Tnl0. J. Bacteriol. 156, 1188-1191. [PDF]

53. Reznikoff, W. S. (1983). Some bacterial transposable elements: Their organizations, mechanisms of transposition, and roles in genome evolution. In Gene Function in Prokaryotes, (J. Beckwith, J. Davies and J. Gallant, eds. ), Cold Spring Harbor Laboratory, pp. 229-252.

54. Reznikoff, W. S. (1984). Gene expression in microbes: The lactose operon model system. In The Microbe 1984, part II, (D. P. Kelly and N. G. Carr, eds. ), Cambridge University Press, pp. 195-218.

55. Yu, X. -M., L. Munson, and W. S. Reznikoff. (1984). Molecular cloning and sequence analysis of trp-lac fusion deletions. J. Mol. Biol. 172, 355-362. [PDF]

56. Johnson, R. C. and W. S. Reznikoff. (1984). Copy number control of Tn5 transposition. Genetics 107, 9-18. [PDF]

57. Bertrand, K. P., K. Postle, L. V. Wray, Jr., and W. S. Reznikoff. (1984). Construction of a single-copy promoter vector and its use in analysis of regulation of the transposon Tnl0 tetracycline resistance determinant. J. Bacteriol. 158, 910-919. [PDF]

58. Yu, X. -M. and W. S. Reznikoff. (1984). Deletion analysis of the CAP-cAMP binding site of the Escherichia coli lactose promoter. Nucleic Acids Res. 12, 5449-5464. [PDF]

59. Johnson, R. C. and W. S, Reznikoff. (1984). The role of the IS50R proteins in the promotion and control of Tn5 transposition. J. Mol. Biol. 177, 645-661. [PDF]

60. Munson, L. M., G. D. Stormo, R. L. Niece, and W. S. Reznikoff. (1984). lacZ translation initiation mutations. J. Mol. Biol. 177, 663-683. [PDF]

61. de la Torre, G. C., J. Ortin, E. Domingo, J. Delamarter, B. Allet., J. Davies, K. P. Bertrand, L. V. Wray, Jr., and W. S. Reznikoff. (1984). Plasmid vectors based on Tnl0 DNA: Gene expression regulated by tetracycline. Plasmid 12, 103-110. [PDF]

62. Ventosa, A., F. Rodriguez-Valera, J. S. Poindexter, and W. S. Reznikoff. (1984). Selection for moderately halophilic bacteria by gradual salinity increases. Can. J. Microbiol. 30, 1279-1282.

63. Munson, L. M., W. Mandecki, M. H. Caruthers, and W. S. Reznikoff. (1984). Oligonucleotide mutagenesis of the lacPUV5 promoter. Nucleic Acids Res. 12, 4011-4017. [PDF]

64. Lambert, P. and W. S. Reznikoff. (1985). Use of transcriptional repressors to stabilize the plasmid copy number of transcription fusion vectors. J. Bacteriol. 162, 441-444. [PDF]

65. Yu, X. -M. and W. S. Reznikoff. (1985). Deletion analysis of the Escherichia coli lactose promoter P2. Nucleic Acids Res. 13, 2457-2468.

66. Peterson, M. L. and W. S. Reznikoff. (1985). Properties of lac in vivo and in vitro. An overlapping RNA polymerase binding site within the lactose promoter. J. Mol. Biol. 185, 535-543. [PDF]

67. Peterson, M. L. and W. S. Reznikoff. (1985). Lactose promoter mutation Pr115 activates an overlapping promoter within the lactose control region. J. Mol. Biol. 185, 525-533. [PDF]

68. Reznikoff, W. S., D. A. Siegele, D. W. Cowing, and C. A. Gross. (1985). The regulation of transcription initiation in bacteria. Ann. Rev. Genet. 19, 355-387. [PDF]

69. Reznikoff, W. S. and W. R. McClure. (1986). E. coli promoters. In "Maximizing Gene Expression"(W. S. Reznikoff and L. Gold, eds. ), Butterworth, Boston, MA, pp. 1-33.

70. Lambert, P. F., D. A. Waring, R. D. Wells, and W. S. Reznikoff. (1986). DNA requirements at the bacteriophage G4 origin of complementary-strand DNA synthesis. J. Virol. 58, 450-458. [PDF]

71. Yu, X. -M. and W. S. Reznikoff. (1986). Deletion analysis of RNA polymerase interaction sites in the Escherichia coli lactose operon regulatory region. J. Mol. Biol. 188, 545-553. [PDF]

72. Krebs, M. P. and W. S. Reznikoff. (1986). Transcriptional and translational sites of IS50. Control of transposase and inhibitor expression. J. Mol. Biol. 192, 781-791. [PDF]

73. Lambert, P. F., E. Kawashima, and W. S. Reznikoff. (1987). Secondary structure at the bacteriophage G4 origin of complementary strand DNA synthesis: in vivo requirement. Gene 53, 257-264. [PDF]

74. Donnelly, C. E. and W. S. Reznikoff. (1987). Mutations in the lac P2 promoter. J. Bacteriol. 169, 1812-1817. [PDF]

75. Yin, J. C. -P. and W. S. Reznikoff. (1987). dnaA, an essential host gene, and Tn5 transposition. J. Bacteriol. 169, 4637-4645. [PDF]

76. Crowell, D. N., W. S. Reznikoff, and C. R. H. Raetz. (1987). The biosynthesis of gram-negative endotoxin: Nucleotide sequence of the Escherichia coli gene for lipid A disaccharide synthase. J. Bacteriol. 169, 5727-5734. [PDF]

77. Cornwell, T. L., S. L. Adhya, W. S. Reznikoff, and P. A. Frey. (1987). The nucleotide sequence of the gal T gene of Escherichia coli. Nucleic Acids Res. 15, 8116. [PDF]

78. Reznikoff, W. S., K. Bertrand, C. Donnelly, M. Krebs, L. E, Maquat, M. Peterson, L. Wray, J. Yin, and X. -M. Yu. (1987). Complex promoters. In "RNA Polymerase and the Regulation of Transcription"(W. S. Reznikoff, R. R. Burgess, J. E. Dahlberg, C. A. Gross, M. T. Record, Jr. and M. P. Wickens, eds. ), Elsevier, New York, pp. 105-113.

79. Yin, J. C. -P., M. P. Krebs, and W. S. Reznikoff. (1988). The effect of dam methylation on Tn5 transposition. J. Mol. Biol. 199, 35-46. [PDF]

80. Kendrick, K. E. and W. S. Reznikoff. (1988). Transposition of IS50L activates downstream genes. J. Bacteriol. 170, 1965-1968. [PDF]

81. Makris, J. C., P. L. Nordmann. and W. S. Reznikoff. (1988). Mutational analysis of IS50 and Tn5 ends. Proc. Natl. Acad. Sci. USA 85, 2224-2228. [PDF]

82. Krebs, M. P. and W. S. Reznikoff. (1988). Use of a Tn5 derivative that creates lacZ translational fusions to obtain a transposition mutant. Gene 63, 277-285. [PDF]

83. Yin, J. C. -P. and W. S. Reznikoff. (1988). p2 and the inhibition of Tn5 transposition. J. Bacteriol. 170, 3008-3015. [PDF]

84. Nordmann, P. L., J. C. Makris, and W. S. Reznikoff. (1988). Inosine induced mutations. Mol. Gen. Genet. 214, 62-67.

85. Makris, J. C., P. L. Nordmann, and W. S. Reznikoff. (1988). Saturation mutagenesis using double-stranded oligonucleotides containing complementary mixed-sequence single strands. DNA and Protein Engineering Techniques 1, 36-38.

86. Field, T. L., W. S. Reznikoff, and P. A. Frey. (1989). Galactose-1-phosphate uridylyltransferase: Identification of His-164 and His-166 as critical residues by site-directed mutagenesis. Biochemistry 28, 2094-2099. [PDF]

87. Karls, R., V. Schulz, S. B. Jovanovich, S. Flynn, A. Pak, and W. S. Reznikoff. (1989). Pseudorevertants of a lac promoter mutation reveal overlapping nascent promoters. Nucleic Acids Res. 17, 3927-3949. [PDF]

88. Makris, J. C. and W. S. Reznikoff. (1989). Orientation of IS50 transposase gene and transposition. J. Bacteriol. 171, 5212-5214. [PDF]

89. Reznikoff, W. S. (1989). Regulation of bacterial gene expression. In "Bacteria in Nature, Vol. 3"(J. S. Poindexter and E. R. Leadbetter, eds. ), Plenum, New York, pp. 337-358.

90. Schulz, V. and W. S. Reznikoff. (1990). In vitro secondary structure analysis of mRNA from lacZ translation initiation mutants. J. Mol. Biol. 211, 427-445. [PDF]

91. Makris, J. C., P. L. Nordmann. and W. S. Reznikoff. (1990). Integration host factor plays a role in IS50 and Tn5 transposition. J. Bacteriol. 172, 1368-1373. [PDF]

92. Kiley, P. and W. S. Reznikoff. (1991). fnr mutants that activate gene expression in the presence of oxygen. J. Bacteriol. 173, 16-22. [PDF]

93. Xiong, X., N. de la Cruz, and W. S. Reznikoff. (1991). Downstream deletion analysis of the lac promoter. J. Bacteriol. 173, 4570-4577. [PDF]

94. Eschenlauer, A. C. and W. S. Reznikoff. (1991). Escherichia coli catabolite gene activator protein mutants defective in positive control of lac operon transcription. J. Bacteriol. 173, 5024-5029. [PDF]

95. Schulz, V. and W. S. Reznikoff. (1991). Translation initiation of IS50R read-through transcripts. J. Mol. Biol. 221, 65-80. [PDF]

96. Choe, M. and W. S. Reznikoff. (1991). Anaerobically expressed Escherichia coli genes identified by operon fusion techniques. J. Bacteriol. 173, 6139-6146. [PDF]

97. Weinreich, M. D., J. C. Makris, and W. S. Reznikoff. (1991). Induction of the SOS response in Escherichia coli inhibits Tn5 and IS50 transposition. J. Bacteriol. 173, 6910-6918. [PDF]

98. Reznikoff, W. S. (1991). Prospectives and challenges in genetic engineering. In Recombinant DNA Technology I, (Prokop and Bajpai, eds. ), Ann. NY Acad. Sci. 646, 1-4.

99. Reznikoff, W. S. (1992). Catabolite gene activator protein activation of lac transcription. J. Bacteriol. 174, 655-658. [PDF]

100. Wiegand, T. W. and W. S. Reznikoff. (1992). Characterization of two hypertransposing Tn5 mutants. J. Bacteriol. 174, 1229-1239. [PDF]

101. Weinreich, M. D. and W. S. Reznikoff. (1992). Fis plays a role in Tn5 and IS50 transposition. J. Bacteriol. 174, 4530-4537. [PDF]

102. Reznikoff, W. S. (1992). The lactose operon controlling elements: a complex paradigm. Mol. Microbiol. 6, 2419-2422.

103. Reznikoff, W. S., R. Jilk, M. P. Krebs, J. C. Makris, P. L. Nordmann, M. Weinreich, and T. Wiegand. (1993). Tn5 lacZ translation fusion elements: Isolation and analysis of transposition mutants. In Recombinant DNA: Methods in Enzymology, (Wu, ed. ), Academic Press 217, pp. 312-322.

104. Choe, M. and W. S. Reznikoff. (1993). Identification of the regulatory sequence of anaerobically expressed locus aeg-46. 5. J. Bacteriol. 175, 1165-1172. [PDF]

105. Jilk, R. A., J. C. Makris, L. Borchardt, and W. S. Reznikoff. (1993). Implications of Tn5 associated adjacent deletions. J. Bacteriol. 175, 1264-1271. [PDF]

106. Kao, C., P. Hauser, W. S. Reznikoff, and C. A. Reznikoff. (1993). Simian Virus 40 (SV40) T-antigen mutations in tumorigenic transformation of SV40-immortalized human uroepithelial cells. J. Virol. 67, 1987-1995. [PDF]

107. Xiong, X. and W. S. Reznikoff. (1993). Transcriptional slippage during the transcription initiation process at a mutant lac promoter in vivo. J. Mol. Biol. 231, 569-580. [PDF]

108. Kao, C., S. Q. Wu, S. DeVries, W. S. Reznikoff, F. M. Waldman, and C. A. Reznikoff. (1993). Carcinogen-induced amplification of SV40 DNA inserted at 9ql2-21. 1 associated with chromosome breaks, deletions, and translocations in human uroepithelial cell transformation in vitro. Genes, Chromosomes and Cancer 8, 155-166.

109. Reznikoff, W. S. (1993). The Tn5 transposon. Ann. Rev. Microbiol. 47, 945-963. [PDF]

110. de la Cruz, N. B., M. D. Weinreich, T. W. Wiegand, M. P. Krebs, and W. S. Reznikoff. (1993). Characterization of the Tn5 transposase and inhibitor proteins: A model for the inhibition of transposition. J. Bacteriol. 175, 6932-6938. [PDF]

111. Wiegand, T. W. and W. S. Reznikoff. (1994). Interaction of Tn5 transposase with the transposon termini. J. Mol. Biol. 235, 486-495. [PDF]

112. Weinreich, M. D., L. Mahnke-Braam, and W. S. Reznikoff. (1994). A functional analysis of the Tn5 transposase: Identification of domains required for DNA binding and multimerization. J. Mol. Biol. 241, 166-177. [PDF]

113. Weinreich, M. D., H. Yigit, and W. S. Reznikoff. (1994). Overexpression of the Tn5 transposase in E. coli results in filamentation, aberrant nucleoid segregation and cell death: Analysis of E. coli and transposase suppressor mutations. J. Bacteriol. 176, 5494-5504. [PDF]

114. Goryshin, I. Y., Y. V, Kil, and W. S. Reznikoff. (1994). DNA length, bending and twisting constraints on IS50 transposition. Proc. Nat. Acad. Sci. USA 91, 10834-10838. [PDF]

115. Weinreich, M. D., A. Gasch, and W. S. Reznikoff. (1994). Evidence that the cis preference of the Tn5 transposase is caused by non-productive multimerization. Genes & Develop. 8, 2363-2374.

116. Tavormina, P., W. Reznikoff, and C. A. Gross. (1996). Identifying interacting regions in the β subunit of Escherichia coli RNA polymerase. J. Mol. Biol. 258, 213-223. [PDF]

117. Jilk, R. A., D. York. and W. S. Reznikoff. (1996). The organization of the outside end of the transposon Tn5. J. Bacterol. 178, 1671-1679. [PDF]

118. Record, M. T., Jr., W. S. Reznikoff, and P. J, Schlax. (1996). Escherichia coli RNA polymerase (Esigma70), promoters, and the kinetics of the steps of transcription initiation. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, 2nd ed., (Neidhardt, Curtiss, Ingraham, Lin, Low, Magasanik, Reznikoff, Riley, Schaechter and Umbarger, eds. ), American Society for Microbiology, pp. 792-820.

119. Schaechter, M., R. Curtiss, III, J. L. Ingraham, E. C. C. Lin, K. B. Low, Jr., B. Magasanik, F. C, Neidhardt, W. Reznikoff, M. Riley, and H. E, Umbarger. (1996). The view from here. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology,2nd ed., (Neidhardt, Curtiss, Ingraham, Lin, Low, Magasanik, Reznikoff, Riley, Schaechter and Umbarger, eds. ), American Society for Microbiology, pp. 2817-2822.

120. York, D. and W. S. Reznikoff. (1996). Purification and biochemical analysis of a monomeric form of Tn5 transposase. Nucleic Acids Res. 24, 3790-3796. [PDF]

121. Czarniecki, D., R. J. Noel, Jr., and W. S. Reznikoff. (1997).  The -45 region of the Escherichia coli lac promoter: CAP-dependent and CAP-independent transcription. J. Bacteriol. 179, 423-429. [PDF]

122. Yigit, H. and W. S. Reznikoff. (1997).  Examination of the Tn5 transposase overproduction phenotype in Escherichia coli and localization of a suppressor of transposase overproduction killing that is an allele of rpoH. J. Bacteriol. 179, 1704-1713. [PDF]

123. York, D. and W. S. Reznikoff. (1997).  DNA-binding and phasing analyses of Tn5 transposase and a monomeric variant. Nucleic Acids Res. 25, 2153-2160. [PDF]

124. Zhou, M. and W. S. Reznikoff. (1997).  Tn5 transposase mutants that alter DNA binding specificity. J. Mol. Biol. 271, 362-373. [PDF]

125. Zhou, M., A. Bhasin, and W. S. Reznikoff. (1998).  Molecular genetic analysis of transposase-end sequence recognition: Cooperation of three adjacent base pairs in specific interaction with a mutant Tn5 transposase. J. Mol. Biol. 276, 913-925. [PDF]

126. Goryshin, I. Y. and W. S. Reznikoff. (1998).  Tn5 in vitro transposition. J. Biol. Chem. 273, 7367-7374. [PDF]

127. York, D., K. Welch, I. Y. Goryshin, and W. S. Reznikoff. (1998).  Simple and efficient generation in vitro of nested deletions and inversions: Tn5 intramolecular transposition. Nucleic Acids Res. 26, 1927-1933. [PDF]

128. Braam, L. M. and W. S. Reznikoff. (1998). Functional characterization of the Tn5 transposase by limited proteolysis. J. Biol. Chem. 273, 10908-10913. [PDF]

129. Noel, R. J., Jr. and W. S. Reznikoff. (1998).  CAP, the -45 region, and RNA polymerase: Three partners in transcription initiation at lacP1 in Escherichia coli. J. Mol. Biol. 282, 495-504. [PDF]

130. Goryshin, I. Y., J. A. Miller, Y. V. Kil, V. A. Lanzov, and W. S. Reznikoff. (1998). Tn5/IS50 target recognition. Proc. Nat. Acad. Sci. U. S. A. 95, 10716-10721. [PDF]

131. Yigit, H. and W. S. Reznikoff. (1998). Escherichia coli DNA topoisomerase I and suppression of killing by Tn5 transposase overproduction: topoisomerase I modulates Tn5 transposition. J Bact. 180, 5866-5874. [PDF]

132. Braam, L. M. and W. S. Reznikoff. (1999). DNA transposition: Classes and mechanisms. In Embryonic Encyclopedia of Life Sciences, Nature Publishing Group, London. www. els. net. [PDF]

133. Braam, L. M., I. Y. Goryshin, and W. S. Reznikoff. (1999). The mechanism of Tn5 inhibition Carboxyl-terminal dimerization. J. Biol. Chem. 274, 86-92. [PDF]

134. Davies, D. R., L. M. Braam, W. S. Reznikoff, and I. Rayment. (1999). The three-dimensional structure of a Tn5 transposase-related protein determined to 2. 9Å resolution. J. Biol. Chem. 274, 11904-11913. [PDF]

135. Yigit, H. and W. S. Reznikoff. (1999). Escherichia coli DNA topoisomerase I copurifies with Tn5 transposase, and Tn5 transposase inhibits topoisomerase I. J. Bacteriol. 181, 3185-3192. [PDF]

136. Bhasin, A., I. Y. Goryshin, and W. Reznikoff. (1999). Hairpin Formation in Tn5 transposition. J. Biol. Chem. 274, 37021-37029. [PDF]

137. Reznikoff, W. S., A. Bhasin, D. R. Davies, I. Y. Goryshin, T. Naumann, I. Rayment, M. Steiniger-White, and S. S. Twining. (1999). Tn5: A molecular window on transposition. Biochem. Biophys. Res. Commun., 266, 729-734. [PDF]

138. Goryshin, I. Y., J. Jendrisak, L. Hoffman, R. Meis, and W. S. Reznikoff. (2000). Insertional transposon mutagenesis by electroporation of released Tn5 transposition complexes. Nature Biotechnology. 18, 97-100. [PDF]

139. Noel, R. J., Jr. and W. S. Reznikoff. (2000). Structural studies of lacUV5-RNA polymerase interactions in vitro: Ethylation interference and missing nucleoside analysis. J. Biol. Chem. 275, 7708-7712. [PDF]

140. Steiniger-White, M. and W. S. Reznikoff. (2000). The C-terminal alpha helix of Tn5 transposase is required for synaptic complex formation. J. Biol. Chem. 275, 23127-23133. [PDF]

141. Davies, D. R., I. Y. Goryshin, W. S. Reznikoff, and I. Rayment. (2000). Three-dimensional structure of the Tn5 synaptic complex transposition intermediate. Science 289, 77-85. [PDF]

142. Naumann, T. A. and W. S. Reznikoff. (2000). Trans catalysis in Tn5 transposition. Proc. Natl. Acad. Sci. USA. 97 8944-8949. [PDF]

143. Bhasin, A., I. Y. Goryshin, M. Steiniger-White, D. York, and W. S. Reznikoff. (2000). Characterization of a Tn5 pre-cleavage synaptic complex. J. Mol. Biol. 302, 49-63. [PDF]

144. Hoffman, L. M., J. J. Jendrisak, R. J. Meis, I. Y. Goryshin and W. S. Reznikoff. (2000). Transposome insertional mutagenesis and direct sequencing of microbial genomics. Genetica 108, 19-24.

145. Twining, S. S., I. Y. Goryshin, A. Bhasin, and W. S. Reznikoff. (2001)Functional characterization of arginine 30, lysine 40 and arginine 62 in Tn5 transposase. J. Biol. Chem. 276, 23135-23143. [PDF]

146. Reznikoff, W. S. (2002). Tn5 transposition. In Mobile DNA II. (N. Craig, R. Craigie, M. Gellert, A. Lambowitz, eds. ), American Society of Microbiology, Washington, D. C. pp. 403-421. [PDF]

147. Naumann, T. A. and W. S. Reznikoff, (2002)Tn5 transposase with an altered specificity to transposon ends. J. Bacteriol. 184, 233-240. [PDF]

148. Ason, B. and W. S. Reznikoff (2002). Mutational analysis of the base flipping event found in Tn5 tranposition. J. Biol. Chem., 277, 11284-11291. [PDF]

149. Lovell, S., I. Y. Goryshin, W. S. Reznikoff, and I. Rayment. (2002)Two metal active site binding of a Tn5 transposase synaptic complex. Nature Structural Biology. 9, 278-281. [PDF]

150. Naumann, T. A. and W. S. Reznikoff. (2002)Tn5 transposase active site mutants. J. Biol. Chem., 277, 17623-17629. [PDF]

151. Steiniger-White, M., A. Bhasin, S. Lovell, I. Rayment, and W. S. Reznikoff. (2002)Evidence for "unseen" transposase-DNA contacts. J. Mol. Biol, 322, 971-982. [PDF]

152. Naumann, T. A., I. Y. Goryshin, and W. S. Reznikoff (2002)Production of combinatorial libraries of fused genes by sequential transposition reactions. Nucleic Acids Res, 30, e119. [PDF]

153. Reznikoff, W. S. (2002). Molecular basis of Tn5 transposition. In Molecular Genetics Biophysics and Medicine Today, Bresler Memorial Lectures, Russian Academy of Sciences, St. Petersburg Scientific Center RAS, B. P. Konstantinov Petersburg Nuclear Physics Institute RAS, 292-297. [PDF]

154. Peterson, G. and W. S. Reznikoff (2003). Tn5 transposase active site mutations suggest position of donor backbone DNA in synaptic complex. J. Biol. Chem. 278, 1904-1909. [PDF]

155. Goryshin, I. Y., T. A. Naumann, J. Apodaca, and W. S. Reznikoff (2003). Chromosomal deletion formation system based on Tn5 double transposition: Use for making minimal genomes and essential gene analysis. Genome Research. 13, 644-653. [PDF]

156. Reznikoff, W. S. (2003). Tn5 as a model for understanding DNA transposition. Molecular Microbiology 47, 1199-1206. [PDF]

157. Ason, B. and W. S. Reznikoff (2004). DNA sequence bias during Tn5 transposition. J. Mol. Biol. 335, 1213-1225. [PDF]

158. Reznikoff, W. S., I. Y. Goryshin, and J. J. Jendrisak, (2004). Tn5 as a molecular genetic tool: In vitro transposition and coupling in vitro technologies with in vivo transposition. In Mobile Genetic Elements: Protocols and Genomic Applications, Methods in Molecular Biology series. (eBook) (W. J. Miller and P. Capy), Humana Press, Inc. Totowa, NJ, Vol. 260, pp. 83-96. [PDF]

159. Steiniger-White, M., I. Rayment, and W. S. Reznikoff (2004). Structure/function insights into Tn5 transposition. Current Opinion in Structural Biology, 14, 50-57. [PDF]

160. Ason, B. and W. S. Reznikoff (2004). A high-throughput assay for Tn5 Tnp induced DNA cleavage. Nucleic Acids Research. 32(10), e83. [PDF]

161. Reznikoff, W. S., S. R. Bordenstein, and J. Apodaca (2004)Comparative sequence analysis of IS50/Tn5 transposase. J. Bacteriol., 186, 8240-8247. [PDF]

162. Winterberg, K. M., J. Luecke, A. Bruegl and W. S. Reznikoff (2005)Phenotypic screening of Escherichia coli K-12 Tn5 insertion libraries using whole genome oligonucleotide microarrays, Applied and Environmental Microbiology, 71, 451-459. [PDF]

163. Ason, B., D. J. Knauss, A. M. Balke, G. Merkel, A. M. Skalka and W. S. Reznikoff (2005)Targeting Tn5 transposase identifies Human Immunodeficiency Virus Type 1 inhibitors. Antimicrobial Agents and Chemotherapy, 49, 2035-2043. [PDF]

164. Bordenstein, Seth R. and W. S. Reznikoff (2005)Mobile DNA in Obligate Intracellular Bacteria, Nature Reviews Microbiology, 3, 688-699. [PDF]

165. Reznikoff, W. S. (2006)Tn5 transposition: A molecular tool for studying protein structure-function. Biochemical SocietyTransactions. 34, 320-323. [PDF]

166. Steiniger, M., C. D. Adams, J. F. Marko, and W. S. Reznikoff. (2006). Defining characteristics of Tn5 transposase non-specific DNA binding. Nucleic Acids Res. 34, 2820-2832. [PDF]

167. Adams, C. D., B. Schnurr, D. Skoko,J. F. Marko, and W. S. Reznikoff. (2006). Tn5 Transposase Loops DNA in the Absence of Tn5 Transposon End Sequences. Molecular Microbiology. 62, 1558-1568. [PDF]

168. Steiniger, M., J. Metzler, and W. S. Reznikoff. (2006). Mutation of Tn5 transposase β-loop residues affects all steps of Tn5 transposition: the role of conformational changes in tn5 transposition. Biochemistry, 45, 15552-15562. [PDF]

169. Winterberg, K. M. and W. S. Reznikoff. (2007). Screening Transposon Mutant Libraries Using Full-Genome Oligonucleotide Microarrays. In Advanced Bacterial Genetics: Use of Transposons and Phage for Genomic Engineering, Methods in Enzymology Series, (Kelly T. Hughes and Stanley R. Maloy, Eds. ) Elsevier Inc., San Diego, CA. Vol. 421, pp. 110-125. [PDF]

170. Winterberg, K. M. and W. S. Reznikoff. (2007). Transposon-based strategies for the identification of essential bacterial genes. In Gene Essentiality at Genome Scale: Protocols and Bioinformatics, (A. Osterman and S. Gerdes, Eds. ) Humana Press, Totowa, NJ. In Press.

171. Adams, C. D., B. Schnurr, J. F. Marko, and W. S. Reznikoff (2007). Pulling apart catalytically active Tn5 synaptic complexes using magnetic tweezers. J. Mol. Biol. 367, 319-327. [PDF]

172. Vaezeslami, S., R. Sterling, and W. S. Reznikoff. (2007). Site-directed mutagenesis studies of the Tn5 transposase residues involved in synaptic complex formation. J. Bacteriology. 189, 7436-7441. [PDF]

173. Czyz, A., K. A. Stillmock, D. J. Hazuda, and W. S. Reznikoff. (2007). Dissecting Tn5 transposition using HIV-1 integrase diketoacid inhibitors. Biochemistry. 46, 10776-10789. [PDF]

174. Kieft, R., V. Brand, D. K. Ekanayake, K. Sweeney, C. DiPaolo, W. S. Reznikoff, and R. Sabatini. (2007). JBP2, a SW12/SNF2-like protein, regulates de novo telomeric DNA glycosylation in bloodstream form Trypanosoma bruccei. Mol. Biochem. Parasitol. 156, 24-31. [PDF]

175. Gradman, R. J. and W. S. Reznikoff. (2008). Tn5 synaptic complex formation: role of transposase residue w450. J. Bact. 190, 1484-1487. [PDF]

176. Gradman, R. J., J. L. Ptacin, A. Bhasin, W. S. Reznikoff, and I. Y. Goryshin. (2008).  A bifunctional DNA binding region in Tn5 transposase. Mol. Microbiol. 67, 528-540. [PDF]

Books

J. H. Miller and W. S. Reznikoff, eds. "The Operon" (1978 and 1980). Cold Spring Harbor Laboratory.

W. S. Reznikoff and L. Gold, eds. "From Gene to Protein: Steps Dictating the Maximal Level of Gene Expression" (1986). Butterworth.

W. S. Reznikoff, R. R. Burgess, J. E. Dahlberg, C. A. Gross, M. T. Record, Jr. and M. P. Wickens, eds. "RNA Polymerase and the Regulation of Transcription" (1987). Elsevier.

J. E. Davies, D. Davies and W. S. Reznikoff, eds. "Milestones in Biotechnology" (1992). Butterworth-Heinemann.

F. C. Neidhardt, R. Curtiss, III, J. L. Ingraham, E. C. C. Lin, K. B. Low, Jr., B. Magasanik, W. Reznikoff, M. Riley, M. Schaechter and H. E. Umbarger, eds. "Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, 2nd ed. (1996). American Society for Microbiology.

Patents

W. S. Reznikoff and I. Y. Goryshin. System for in vitro transposition. U. S. Patent No. 5,965,443.

W. S. Reznikoff, D. York, I. Y. Goryshin, and H. Zhou. System for in vitro transposition . Patent No. 5,948,622. (CIP)

W. S. Reznikoff, I. Y. Goryshin and H. Zhou. System for in vitro transposition. U. S. Patent No. 5,925,545. (CIP)

W. S. Reznikoff, I. Y. Goryshin, and Hong Zhou, System for in vitro transposition. U. S. Patent No. 6,437,109

W. S. Reznikoff and I. Y. Goryshin. Method for making insertional mutations. U. S. Patent No. 6,159,736

W. S. Reznikoff and T. A. Naumann. Transposase enzyme and method for use. U. S. Patent No. 6,406,896

I. Y. Goryshin and W. S. Reznikoff. Method for making insertional mutations using a Tn5 synaptic complex. U. S. Patent No. 6,294,385. (CIP)

Patents Pending

I. Y. Goryshin, T. A. Naumann and W. S. Reznikoff. Double transposition methods for manipulating nucleic acids. WARF P00359US.

W. S. Reznikoff, M. M. Steiniger-White and J. Metzler. Tn5 transposase mutants and the use thereof. WARF P02358US.

W. S. Reznikoff and R. J. Gradman. Mutated Tn5 transposase proteins and the use thereof. WARF P03381US.

B. L. Ason, W. S. Reznikoff, A. M. Skalka and D. J. Knauss. Modulators of enzymatic nucleic acid transposition. WARF P0409US.