BIOCHEMISTRY

Downloadable/printable pdf version here.

Tuesday, June 7, Discovery Building – 330 N Orchard Street

7:45 AM – 8:30 AM Registration check-in, poster setup, & light breakfast

Opening Session – H.F. DeLuca Forum

8:30 AM – 8:50 AM Brian Fox, UW–Madison & Brad Schwartz, Morgridge Institute for Research —
Welcome and Introductory Remarks

8:50 AM – 9:00 AM Paula Flicker and Mary Ann Wu, National Institutes of Health —
The NIH Common Fund Program for Transformative High-Resolution Cryo-EM

9:00 AM – 9:30 AM Elizabeth Wright, UW–Madison —
Building the Cryo-EM Centers at UW–Madison

SESSION 1 – High-Resolution Cryo-EM Structural Studies of Pathogens – H.F. DeLuca Forum

9:30 AM – 9:50 AM Deb Kelly, Penn State University —
High-Resolution Imaging of SARS-CoV-2 Sub-Viral Assemblies Derived from COVID-19 Patients

9:50 AM – 10:10 AM Susan Lea, National Cancer Institute
Using Protons in the Bacterial Cytoplasmic Membrane

10:10 AM – 10:30 AM Robert Kirchdoerfer, UW–Madison —
Co-Factor Interactions in Alpha and Betacoronavirus Core Polymerase Complexes

10:30 AM – 11:00 AM Morning Break – Atrium

SESSION 2 – Many Small Things Considered by Cryo-EM – H.F. DeLuca Forum

11:00 AM – 11:20 AM Ci Ji Lim, UW–Madison —
Single-Stranded DNA-Binding Protein CST Sets the Stage for Human DNA Polymerase Alpha/Primase RNA-DNA Primer Synthesis

11:20 AM – 11:30 AM Wei Huang, Case Western Reserve University —
Structural and Mechanistic Basis for Recognition of Alternative tRNA Precursor Substrates by Bacterial Ribonuclease P

11:30 AM – 11:40 AM James Letts, UC Davis —
Structures of Tetrahymena’s Respiratory Chain Reveal the Diversity of Eukaryotic Core Metabolism

11:40 AM – 12:00 PM Michael Schmid, Stanford SLAC, S2C2, SCSC —
Tubulin Intra- and Inter-Polymer Interactions in Toxoplasma

12:00 PM – 12:10 PM Xinyun Cao, UW–Madison —
Basis of Narrow-Spectrum Activity of Fidaxomicin on Clostridioides difficile

12:10 PM – 12:30 PM Michael Stowell, CU Boulder —
The Long Pursuit of the Muscle Type nAChR Structure: Using Chemistry to Tackle Biology

12:30 PM – 1:30 PM Lunch in and around the Discovery Building

1:30 PM – 2:30 PM Poster Session & Exhibits – Atrium

2:30 PM – 2:45 PM Afternoon Break – Atrium

2:45 PM Meet in the Discovery Building Atrium to travel to workshops and tours

3:00 PM – 5:00 PM Workshops & Tours – Biochemistry Buildings

5:00 PM – 5:30 PM Break, Return to Discovery Building

5:30 PM – 6:30 PM Keynote Presentation – H.F. DeLuca Forum
David Veesler, University of Washington, HHMI —
Structure-Guided Coronavirus Vaccine Design

6:30 PM– 8:30 PM Dinner – Atrium

Wednesday, June 8, Discovery Building – 330 N Orchard Street

7:45 AM – 8:30 AM Registration check-in & light breakfast

8:30 AM – 9:30 AM Boyer Seminar & Award Presentation – H.F. DeLuca Forum
Jae Yang – UW–Madison
In-situ Cryo-Electron Tomography: Exploring Cellular Machinery at the Nanoscale

9:30 AM – 10:00 AM Morning Break

SESSION 3 – Tools of the Trade: Developments in Methods and Applications of Cryo-EM – H.F. DeLuca Forum

10:00 AM – 10:20 AM Wah Chiu, Stanford SLAC, S2C2, SCSC —
Cryo-EM is a Tool to Answer Long Standing Biochemistry Puzzles

10:20 AM – 10:30 AM Beth Stadtmueller, University of Illinois Urbana-Champaign —
Cryo-Electron Microscopy Structures of Secretory Immunoglobulins from Mammals and Fish Reveal Long Hidden Structure-Function Relationship

10:30 AM – 10:40 AM Vicky Pappas, UW–Madison —
Cryo-EM Structural Studies of the Vibrio cholerae Flagellum

10:40 AM – 10:50 AM Daija Bobe, NYSBC, SEMC, NCITU —
The Waffle Method: An Approach for Cryo-FIB/SEM Thin Lamellae Preparation

10:50 AM – 11:00 AM Brandon Malone, The Rockefeller University —
Structural Insights into Substrate Selection by the SARS-CoV-2 Replicase

11:00 AM – 11:20 AM Tim Grant, Morgridge Institute for Research & UW–Madison —
New Methodologies for Preparing and Imaging Cryo-EM Samples

11:20 AM – 11:30 AM Open Discussion

11:30 AM – 12:30 PM Lunch in and around the Discovery Building

SESSION 4 – Explorations In Situ: Cryo-ET is Coming of Age – H.F. DeLuca Forum

12:30 PM – 12:50 PM Clint Potter, NYSBC, NRAMM, NCCAT, NCITU —
Challenges of Cellular Cryotomography

12:50 PM – 1:00 PM Joseph Kim, UW–Madison —
Morphological Comparisons of Primary Neurons Cryo-Preserved Under Varied Conditions

1:00 PM – 1:20 PM Andreas Hoenger, CU Boulder —
Cryo-ET at CU Boulder: From Microtubules to Cells and Tissues

1:20 PM – 1:30 PM Thomas Laughlin, UC San Diego —
Architecture and Self-Assembly of the Giant Bacteriophage Nucleus-Like Compartment

1:30 PM – 1:40 PM Bryan Sibert, UW–Madison —
Structure of Respiratory Syncytial Virus Matrix Protein Determined with Sub-Nanometer Resolution using Cryo-Electron Tomography

1:40 PM – 2:00 PM Danielle Grotjahn, Scripps Research Institute —
Structure Among the Chaos: Using Cellular Tomography to Study Mitochondrial Behavior

2:00 PM – 2:15 PM Afternoon Break

2:15 PM Meet in the Discovery Building Atrium to travel to workshops and tours

2:30 PM – 4:30 PM Workshops & Tours – Biochemistry Buildings

4:30 PM – 5:00 PM Break, Return to Discovery Building – H.F. DeLuca Forum

5:00 PM – 5:30 PM Elizabeth Wright, UW–Madison –
Closing Remarks & Poster Awards Presentation

5:30 PM – 6:30 PM Refreshments, Posters & Exhibits – Atrium

6:30 PM Take posters down

Workshops

If you are interested in attending any of the workshops listed below, please indicate your preferences at the time of registration. To amend your registration, contact Matthew Freid at PLACE: Professional Learning and Community Education cell: 716.553.2654 or email: freid@wisc.edu. Please reference the 42^nd Steenbock Symposium in the subject line of your email.

Workshop 1: Mass is mass! Using the Refeyn mass photometry system for cryo-EM sample optimization

Workshop 2: We must have order! Micropatterning with the Alveole PRIMO system for advanced cryo-CLEM, cryo-FIB, and cryo-ET workflows

Workshop 3: Is that my complex? Advanced cryo-CLEM with the Leica cryo-confocal CLEM or cryo-CLEM systems for accurate correlation in cellular environments

Workshop 4: Can you make it thinner? Cryo-FIB milling cells with the Aquilos 2 cryo-FIB

Workshop 5: Taking my (multi-)shot! Cryo-electron tomography on the Krios G3 and Krios G4