Iron-Sulfur Proteins—Biogenesis, Regulation and Function

39th Steenbock Symposium - May 29, 2018 to Jun 02, 2018
Memorial Union


Meeting Schedule
Tuesday, May 29, 2018
5:00 pm – 7:30 pm Registration and reception (Shannon Sunset Lounge, Memorial Union)
Excursion sign-up
7:30 pm – 8:30 pm Keynote lecture: (Shannon Hall) (Chair: John Markley)
Roland Lill: Biogenesis of Iron-Sulfur Proteins in Eukaryotes: Components, Mechanisms, and Diseases
8:30 pm – 10:00 pm Cash bar on Sunset Deck-Upper:
Wednesday, May 30, 2018
Session 1: 9:00 am – 10:30 am (chair: Perry Frey)
9:00 am             Joan Broderick: A New Paradigm for Radical SAM Reactions
9:30 am             Squire Booker: A Saga of Death, Destruction, and Rebirth: the Role of Iron-Sulfur Clusters in the Biosynthesis of Lipoic Acid
10:00 am          Catharina M. Blinn: Apd1 and Aim32 are Prototypes of a New Class of [2Fe-2S] Proteins
10:15 am          Vikas Nanda: Design of a Primordial Ferredoxin
Break: 10:30 – 11:00 am
Session 2: 11:00 am – 12:30 pm (chair:  Dennis Dean)
11:00 am          Lance Seefeldt: Energy Transduction in Nitrogenase
11:30 am          Brian Hoffman: The Mechanism of Nitrogen Fixation by Nitrogenase
12:00 n             Lightning poster talks (odd numbered)
Lunch: 12:30 pm – 2:00 pm (food served outside Shannon Hall, take to Tripp Commons/Deck; dessert in Tripp)
Poster session 1: 2:00 pm – 4:00 pm (Tripp Commons)
Session 3: 4:00 pm – 6:00 pm (chair: Patricia Dos Santos)
4:00 pm             Yasuhiro Takahashi: Novel Insights into Bacterial Iron–Sulfur Cluster Biosynthesis Systems
4:30 pm             Sandrine Ollagnier: Mechanistic Investigations on the Fe-S Cluster Assembly by the SUF Machinery
5:00 pm             F. Wayne Outten: Exploring Interactions between the Suf Fe-S Cluster Biogenesis Pathway, Fe-S Cluster Trafficking Networks, and Iron Homeostasis in E. coli
5:30 pm             Patrick A. Frantom: Changes in Protein Dynamics in Escherichia coli SufS Reveal a Possible Conserved Regulatory Mechanism in Type II Cysteine Desulfurase Systems
5:45 pm             Vojtech Vacek: Fe-S Cluster Assembly in Oxymonads and Related Protists              
Thursday, May 31, 2018
Session 4: 9:00 am – 10:30 am (chair: Timothy Stemmler)
9:00 am             Miroslav Cygler: Structure and Dynamics of the Core Fe/S Cluster Assembly Complex
9:30 am             Marco Tonelli: Fluorine-19 NMR Investigations of Steps in Iron-Sulfur Cluster Assembly
9:45 am             Hélène Puccio: Therapeutic Development for Friedreich Ataxia: Replacing or Bypassing Frataxin?
10:15 am          Paul Lindahl: Analysis of Mrs3ΔMrs4Δ and Yfh1Δ Saccharomyces cerevisiae, and the Role of Fe580
Break: 10:30 – 11:00 am
Session 5: 11:00 am – 12:30 pm (chair: Jin Hae Kim)
11:00 am          David Barondeau: Elucidating Control Mechanisms for the Eukaryotic Fe-S Cluster Biosynthetic Complex
11:30 am          Dennis Winge: The Mitochondrial Acyl Carrier Protein Acp1 Coordinates Mitochondrial Fatty Acid Synthesis with Iron Sulfur Cluster Biogenesis
11:45 am          Trey Sato: Directed Evolution uncovers Mutations in Fe-S Cluster Biogenesis Genes that Enable Anaerobic Xylose Fermentation by Saccharomyces cerevisiae
12:15 pm          Benoit D'autréaux:  Insight into the Primary Steps of Fe-S Cluster Assembly by the ISC Machinery
Box Lunches: 12:30 pm (food can be eaten on the Terrace or in Tripp Commons)
Free afternoon and evening

Friday, June 1, 2018
Session 6: 9:00 am – 10:30 am (chair: Joff Silberg)
9:00 am             Lucia Banci: Structural and Functional Characterization of Processes for FeS protein Biogenesis
9:30 am             Jaroslaw Marszalek: Hsp70 Chaperone Systems in Iron-Sulfur Cluster (FeS) Biogenesis
10:00 am          Ian Campbell: Evolutionary Relationships between Low Potential Electron Carriers that use 2Fe-2S, 4Fe-4S, and Flavin Mononucleotide Cofactors
10:15 am          Giovanna Ghirlanda: Artificial Iron-Sulfur Proteins for Electron Transfer and Catalysis
Break: 10:30 – 11:00 am
Session 7: 11:00 am – 12:30 pm (chair: Brian Fox)
11:00 am          Jared Rutter: Coordinate Regulation of OXPHOS Biogenesis
11:30 am          Nicolas Rouhier: Functional Characterization of Mitochondrial NFU Proteins in Plants
12:00 n              Lightning poster talks (even numbered)
Lunch: 12:30 pm – 2:00 pm
  Committee meeting: CapView Room, 4th floor Memorial Union
Poster session 2: 2:00 pm – 4:00 pm
Session 8: 4:00 pm – 6:00 pm (chair: Beatrice Py)
4:00 pm             Dennis Dean: Sequential and Differential Interaction of Assembly Factors during Nitrogenase MoFe Protein Maturation
4:30 pm             Michael K. Johnson: Scaffold-Free Fe-S Cluster Assembly using a Thioferrate-Protein Nanoparticle
4:45 pm             Silke Leimkühler: Shared Sulfur Mobilization Routes for Fe-S Cluster Assembly and Molybdenum Cofactor
Biosynthesis in Escherichia coli

5:15 pm             Frédéric Barras: FeS under Stress: Acting at all Stages of Production
5:45 pm             Patricia Dos Santos: Redox Regulation involving Bacillus subtilis Thioredoxin and Fe-S Clusters
Saturday, June 2, 2018
Session 9: 9:00 am – 10:30 am (chair: Jeff Boyd)
9:00 am             Nick Le Brun: Studies of Iron-Sulfur Cluster Reactivity by Mass Spectrometry
9:30 am             Jim Imlay: Iron-Sulfur Enzymes Lie at the Heart of the Oxygen Sensitivity of an Obligately Anaerobic Bacterium           
10:00 am          Rebekah M.B. Silva: UvrC Coordinates a Redox-Active 4Fe-4S Cluster Critical for Stability and Function
10:15 am          Dror Noy: CCIS1, a Computationally Designed Protein Expressed in E. coli, and Assembled in vivo as an Iron Sulfur Cluster with a Non-natural Fold
Break: 10:30 – 11:00 am
Session 10: 11:00 am – 12:30 pm (chair: Rick Eisenstein)
11:00 am          Deborah Perlstein: CIA Secrets Revealed: Dre2 and Nar1 Collaborate with the Nbp35-Cfd1 Scaffolding Complex During the Early Steps of Cytosolic Iron Sulfur Cluster Assembly
11:30 am          P. Ryan Potts: Cytosolic Iron-Sulfur Assembly is Evolutionarily Tuned by a Cancer-Amplified Ubiquitin Ligase
11:45 am          Carina Greth: Molecular Determinants for Cytosolic Iron-Sulfur Cluster Insertion
12:00 n              Joseph J. Braymer: The Contribution of Thiol Redox Chemistry to Mitochondrial and Cytosolic Iron-Sulfur Cluster Biogenesis
12:15 pm          Xiaokang Wang: A linker-Targeted sgRNA Reveals Canonical and Non-canonical Function of DRE2
Lunch: 12:30 pm Box lunches (food can be eaten on the Terrace or in Tripp Commons)
Poster session 3: 2:00 pm – 3:30 pm (Tripp Commons)
Remove posters: 3:30
Session 11: 4:00 pm – 6:00 pm (chair: George Reed)
4:00 pm             Caroline C Philpott: Distributing Iron in Cells: BolA2 Links PCBP1-Bound Ferrous Iron to the 2Fe-2S Distribution System
4:15 pm             Jorge L. Porfido: Unique Features of an Unusual Parasite Thioredoxin     
4:30 pm             Rick Eisenstein: A Synergistic Role of Iron Regulatory Protein 1 and FBXL5 in Coordinating Iron Metabolism During Cell Proliferation
4:45 pm            Elizabeth A. Leibold: Iron Regulatory Protein 2 Regulates Insulin Production in Pancreatic β-Cells through Iron-Mediated Translation
5:00 pm – 6:00 pm Keynote lecture (chair: Tricia Kiley)
Tracey Rouault: Recent progress on iron sulfur proteins, ubiquitous and indispensable contributors to metabolism in all kingdoms of life

7:00 pm – 9:30 pm Banquet: (buffet style Tripp Commons)
9:00 pm – 11:00 pm Cash bar on Sunset Deck-Upper
Sunday, June 3, 2018: departure