Fe-S Proteins: Biogenesis, Structure and Function

31st Steenbock Symposium - May 19, 2005 to May 22, 2005


Activities at the Pyle Center, 702 Langdon Street, Madison, Wisconsin

Thursday, May 19, 2005

4:30pm - 6:30pm  - Reception and registration - Pick up conference materials.

Session #1 - Biogenesis of Fe-S Clusters - 1 - Chair, Patricia Kiley

6:45pm - Welcome

7:00pm - 7:30pm - Biochemical-Genetic Analysis of Fe-S Cluster Biosynthesis in Azotobacter vinelandii [PDF]
Dennis Dean - Virginia Tech

7:30pm - 8:00pm - The Roles of Chaperones HscA and HscB in Fe-S Protein Biogenesis [PDF]
Larry Vickery - U. California-Irvine

​8:00pm - 8:20pm - Multiple Turnover 2Fe-2S Cluster Transfer Activity of IscA and IscU [PDF]
​Francesco Bonomi - University of Milan

​8:20pm - 8:40pm - Break

8:40pm - 9:10pm - Structure-Function and Evolutionary Aspects of Fe-S Cluster Assembly Systems [PDF]
Yasuhiro Takahashi - Osaka University

9:10pm - 9:30pm - The First Fe-S Cluster-Bound Crystal Structure of a Scaffold Protein [PDF]
Masato Nakai - Osaka University

9:30pm - 9:50pm - Iron Binding in IscA and Iron Delivery for the Biogenesis of Iron-Sulfur Clusters [PDF]
Huangen Ding - Louisiana State University

Friday, May 20, 2005

Session #2 - Biogenesis of Fe-S Clusters - II - Chair, Rick Eisenstein

8:30am - 9:00am - Biogenesis of Iron-Sulfur Proteins in Mitochondria and Cytosol of Eukaryotes [PDF]
​Roland Lill - U. Marburg

​9:00am - 9:30am - Cfd1p: A Cytosolic Fe-S Cluster Assembly Factor [PDF]
William Walden - Illinois-Chicago

9:30am - 9:50am - Ssq1-Jac1, a Specialized Chaperone System Involved in Biogenesis of Fe-S Cluster Containing Proteins in Yeast Mitochondria [PDF]
Jaroslaw Marszalek - University of Gdansk

​9:50am - 10:15am - Break

10:15am - 10:45am - Mammalian Iron-Sulfur Biogenesis in Mitochondria and Cytosol [PDF]
Tracey Rouault - NIH

​10:45am - 11:15am - The SUF Machinery in the Iron-Sulfur Cluster Biosynthesis [PDF]
Sandrine Ollagnier-de Choudens - Grenoble

​11:15am - 11:35am - Analysis of Fe-S Cluster Formation in Plant Chloroplasts [PDF]
Marinus Pilon - Colorado State

​11:35am - 11:55am - Analysis of the Heteromeric CsdA-CsdE Cysteine Desulfurase, Assisting Fe-S Biogenesis in Escherichia coli [PDF]
Beatrice Py - UPR-CNRS

12:00pm - 1:30pm - Lunch

1:30pm - 4:15pm - Poster Session 1
The presenters are those on the odd numbered pages of the abstract book.

Session #3 - The Role of Fe-S Clusters in Friedreich Ataxia - Chair, Elizabeth Craig

4:30pm - 5:00pm - Frataxin, Iron, and Oxygen [PDF]
Andrew Dancis - U. Penn

​5:00pm - 5:30pm - Mouse and Cellular Models for Friedreich Ataxia:Consequences of Frataxin Deficiency [PDF]
Helene Puccio - Université Louis Pasteur

​5:30pm - 5:50pm - Iron Binding and Delivery by Frataxin [PDF]
Timothy Stemmler - Wayne State University

​5:50pm - 6:10pm - CyaY: Characterization of Iron Binding [PDF]
Salvatore Adinolfi - The Ridgeway

​6:10pm - 6:30pm - Frataxin Deficiency Alters the Heme Pathway and a Homolog of a Yeast Metallochaperone in Mammalian Cells [PDF]
Gino Cortopassi - UC-Davis

​6:30pm - Dinner - Dinner on your own. Independent evening activities.

Saturday, May 21, 2005

Session #4 - Novel Functions of Fe-S Clusters - Chair, Eckard Münck

8:30am - 9:00am - Chemical Approaches to Complex Heterometal Sites in Biology [PDF]
Richard Holm - Harvard

9:00am - 9:30am - Roles for [4Fe-4S] Clusters in the Repair of Damaged DNA Bases [PDF]
Sheila David - U. Utah

9:30am - 10:00am - Site-Specific Reactivity of [4Fe-4S] Clusters in Disulfide Reductases and Radical SAM Enzymes [PDF]
Michael K. Johnson - U. Georgia

10:00am - 10:30am - Break

10:30am - 11:00am - Iron-Sulfur Clusters in AdoMet-mediated Radical Chemistry [PDF]
Joan Broderick - Michigan State

11:00am - 11:30am - Biosynthesis of the Lipoyl Cofactor. Understanding Sulfur Insertion into Unactivated Alkanes [PDF]
​Squire Booker - Penn State

11:30am - 11:50am - A Role for Iron-Sulfur Cluster Assembly and Repair in Biotin Biosynthesis [PDF]
Joseph Jarrett - University of Pennsylvania

12:00pm - 1:30pm - Lunch

1:30pm - 4:15pm - Poster Session 2
The presenters are those on the even numbered pages of the abstract book.

Session #5 - The Role of Fe-S Clusters in Stress and Human Disease - Chair, Claire Kennedy

4:30pm - 5:00pm - Iron-Sulfur Clusters Confer Vulnerability to Oxidative Stress [PDF]
James Imlay - U. Illinois

​5:00pm - 5:30pm - Crystal Structure of the Aconitase Form of Human IronRegulatory Protein 1 [PDF]
Anne Volbeda - IBS Grenoble

​5:30pm - 5:50pm - Enhanced NO Sensitivity of the Fe-S Cluster inPhosphomimetic Mutants of IRP1/c-aconitase [PDF]
Kathryn Deck - University of Wisconsin-Madison

5:50pm - 6:10pm - Iron Homeostasis Under Stress: Fe-S Cluster Biosynthesis and the suf Pathway in E. coli [PDF]
Wayne Outten - University of South Carolina

​6:10pm - 6:30pm - Coordinating Expression of Fe-S Biogenesis Pathways in Escherichia coli [PDF]
Jennifer Giel - University of Wisconsin-Madison

6:30pm - Reception

7:30pm - Banquet

Sunday, May 22, 2005

Session #6 - Structural Aspects of Fe-S Clusters - Chair, Helmut Beinert​

8:30am - 9:00am - From Madison to Grenoble and Back: Riding Ferredoxins Through Iron-Sulfur Territory [PDF]
Jacques Meyer - Grenoble

9:00am - 9:30am - Characterizing the Invisible: EPR and ENDOR Studies of Intermediates in N2 Reduction by Nitrogenase [PDF]
​Brian Hoffman - Northwestern

9:30am - 9:50am - Living on Sulfur: 3D Architecture and Spectroscopy of the Iron-Sulfur Enzymes APS Reductase and Dissimilatory Sulfite Reductase [PDF]
​Peter Kroneck - Fachbereich Biologie Universität Konstanz

​9:50am - 10:15am - Break

10:15am - 10:45am - The Anatomy of AdoMet Radical Enzymes [PDF]
Catherine Drennan - MIT

10:45am - 11:15am - Lysine 2,3-aminomutase: Correlation of Reduction Potential for the [4Fe-4S] Center with the Crystal Structure
Perry Frey - University of Wisconsin-Madison

11:15am - 11:35am - The H cluster: A Light-Sensitive 6Fe Active Site in [Fe-Fe]-Hydrogenases [PDF]
Simon Albracht - University of Amsterdam

​11:35am - 11:55am - Hydrogenase Structure, Function, and Expression [PDF]
John Peters - Montana State University

12:00pm - Box Lunch