UW-Madison Department of Biochemistry RNA MaxiGroup logo

 

RNA research at Madison thrives in many labs. RNA MaxiGroup is intended to bring those labs together to exchange ideas and methods. Presentations are without slides to encourage discussion.

Anyone interested is welcome, even if they are not particularly interested in RNA in general - graduate students, post-docs, staff and faculty.

Email to join the mailing list.

Lights snacks are provided, beginning at 6:10 pm

Organizers - Marv Wickens (Chair), Dave Brow, Sam Butcher, Jim Dahlberg, Heidi Dvinge, Aaron Hoskins, Judith Kimble, Andrew Mehle, Mike Sheets, Karen Wassarman and Jill Wildonger

Image of mitochondrial functions

Control of many mitochondrial functions through the binding of a single PUF protein to many mRNAs. A multi-omic study integrated RNA tagging, CLIP, proteomics, lipidomics and metabolomics to identify mRNAs controlled by the PUF protein, Puf3p. These include the protein import machinery, the mitochondrial ribosome, and oxidative phosphorylation. Collaboration between Wickens and Pagliarini labs

Figure of single molecule colocalization

Data from the Hoskins Lab depicting single molecule colocalization of the yeast U1 snRNP and Branchpoint Bridging Protein (BBP) during spliceosome E complex formation

Image of new families of nucleotidyl transferase enzymes

New families of nucleotidyl transferase enzymes. The enzyme GLD-2 was discovered in C. elegans through a combination of genetics and biochemistry (collaboration of Kimble and Wickens labs). This led to tethered function assays that revealed families of regulatory enzymes that add A’s (PAPs) or U’s (PUPs) to a wide range of RNAs. Both families have widespread roles in biology, including development, the nervous system, and disease. From the Wickens lab

Image of protein curvature

Protein curvature in a common architecture determines the specificity of RNA-protein interactions. Biochemical, genetic and structural analyses of multiple PUF proteins bound to their RNA targets reveals that the curvature of the proteins is critical in determining which RNAs each protein binds. Wickens and T. Hall labs

Image of U1 snRNP and branchpoint bridging protein

U1 snRNP (red) and branchpoint bridging protein (green) binding events on 30 single molecules of pre-mRNA, seen by Colocalization Single Molecule Spectroscopy. Spliceosome E complex formation is noted by the blue bars. From the Aaron Hoskins lab

Crystal structure of the yeast Prp24-U6 RNA complex

Crystal structure of the yeast Prp24-U6 RNA complex (PDB ID: 4n0t) illustrating the topological entanglement of RNA and protein. From the Dave Brow lab

Image of human cardiomyocyte

Human cardiomyocyte derived from IPS cells. From the Mike Sheets lab

Illustration of hotspot mutations

The Hoskins Lab studies hotspot mutations in the spliceosome protein SF3b1 that cause myelodysplastic syndromes (MDS) and other blood cancers

Image of rewiring of RNA-protein networks

Evolutionary rewiring of RNA-protein networks. Direct biochemical analyses of RNA-protein interactions in two highly diverged fungal species demonstrates that a biological function controlled by Puf3p in one species is controlled by a different protein, Puf5p, in the other. Collaboration between the Wickens and Gasch labs

Image of structure of 5’ UMP

Structure of 5’ UMP bound in the active site of Usb1. From the Sam Butcher lab

Photo of Bicc1 particles in Retinal pigment epithelial cells. From the Mike Sheets lab

Cytoplasmic particles formed by the human Bicaudal-C translational repressor protein

Photo of nematode

RNA regulation drives cell fate decisions in the germ cells in all animals. This general principle emerged from work on the nematode germline, which is pictured here with its stem cells at one end (blue) and gametes at the other (oocytes, green; sperm, red). From the Judith Kimble lab

Structure of U6 RNA

Structure of U6 RNA bound to Prp24 from the Sam Butcher lab

Photo of RNA-binding proteins in neurons

Investigating the function of RNA-binding proteins in neurons. From the Jill Wildonger lab

Image of kymograph

Tracks of machinery that support local translation in dendrites. From the Jill Wildonger lab

Image of the yeast Sen1

Model of the yeast Sen1-dependent termination complex scanning a nascent RNA polymerase II (Pol II) transcript. Binding of terminator elements in the transcript to Nrd1 and Nab3 elicits termination of transcription in a manner dependent on the helicase activity of Sen1 and glutamate 108 of the Rpb11 subunit. From the Dave Brow lab

Photo of Aaron Hoskins and Josh Larson

Aaron Hoskins (left) and Josh Larson (right) use their multi-wavelength, micro-mirror CoSMoS microscope for single molecule studies of spliceosome assembly

Photo of influenza virus genome

The Andrew Mehle Lab “hacked” the influenza virus genome to encode the very small and bright NanoLuciferase. In the example shown here, reporter virus was imaged following infection in an embyronated chicken eggs. Blood vessels and the embryo at the bottom are clearly visible. Artistic license was used to depict virus streaking away from the open top of the infected egg

Image of influenza A reporter viruses

Fluorescent influenza A reporter viruses were designed to enable real-time detection of co-infected cells. Live cell imaging identifies cells infected by both a GFP and RFP reporter viruses. From the Andrew Mehle Lab

Figure of RNA duplex

The Hoskins Lab studies how the spliceosome protein SF3b1 recognizes the RNA duplex formed between the intronic branch site and the U2 snRNA

Figure of RNA-protein interactions

RNA-protein interactions inside a cell identified by “RNA tagging” A chimeric protein consisting of an RNA-binding protein (RBP) fused to a U-adding enzyme (PUP) is expressed in a cell. Binding of that RBP-PUP protein to an mRNA via a binding element (blue rectangle) marks that RNA with U’s. From the Marv Wickens lab

Christine Jacobs-Wagner
Christine Jacobs-Wagner
HHMI Yale University
Seminar Series: RNA MaxiGroup
Host: Wassarman
April 10, 2012 - 6:30pm
Rachel Green
Rachel Green
John Hopkins University
Seminar Series: RNA MaxiGroup
Host: Wassarman
March 13, 2012 - 6:30pm
Jeff Kieft
Jeff Kieft
HHMI, University of Colorado-Denver
Seminar Series: RNA MaxiGroup
Host: Butcher
December 13, 2011 - 6:30pm
Erik Miska
Erik Miska
The Gurdon Institute University of Cambridge, UK
Seminar Series: RNA MaxiGroup
Host: Kennedy
November 8, 2011 - 6:30pm
Alfonso Mondragon
Alfonso Mondragon
Northwestern University
Seminar Series: RNA MaxiGroup
Host: Butcher
October 11, 2011 - 6:30pm
Aaron Hoskins
Aaron Hoskins
University of Wisconsin-Madison
Seminar Series: RNA MaxiGroup
Host: Brow
September 13, 2011 - 6:30pm
Allan Jacobson
Allan Jacobson
University of Massachusetts
Seminar Series: RNA MaxiGroup
Host: Wickens
March 1, 2011 - 6:30pm
James Goodrich
James Goodrich
Seminar Series: RNA MaxiGroup
Host: Wassarman
January 11, 2011 - 6:30pm
Christine Guthrie
Christine Guthrie
University of California-San Francisco
Seminar Series: RNA MaxiGroup
Host: Brow
October 14, 2010 - 6:30pm
Joan Steitz
Joan Steitz
HHMI, Yale University
Seminar Series: RNA MaxiGroup
Host: Wassarman
October 12, 2010 - 6:30pm
Robert Darnell
Robert Darnell
HHMI, The Rockerfeller University
Seminar Series: RNA MaxiGroup
Host: Wickens
September 14, 2010 - 6:30pm
Jamie Williamson
Jamie Williamson
The Scripps Research Institute
Seminar Series: RNA MaxiGroup
September 7, 2010 - 6:30pm
Melissa Jurica
Melissa Jurica
University of California, Santa Cruz
Seminar Series: RNA MaxiGroup
Host: Butcher
May 11, 2010 - 6:30pm
Amy Pasquinelli
Amy Pasquinelli
University of California, San Diego
Seminar Series: RNA MaxiGroup
Host: Kennedy
April 20, 2010 - 6:30pm
John Abelson
John Abelson
University of California, San Francisco
Seminar Series: RNA MaxiGroup
Host: Dahlberg
April 8, 2010 - 6:30pm

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