The Biochemistry Optical Core (BOC) provides state-of-the-art instrumentation for light and fluorescence based microscopy, including epifluorescence, confocal, and super-resolution imaging. Established by a faculty-approved investment from the Department of Biochemistry Endowment, our goal is to serve the campus research community by providing access to and training on basic and advanced light microscopy techniques.  Expertise and advice is available for the design of experiments involving these techniques.  

Open to All Academic and Industrial Researchers

Available Equipment Include

Nikon A1R-Si+ Confocal Microscope

Nikon SIM (Structured Illumination Microscope)

Nikon STORM/TIRF/epifluorescence Microscope

Offline Nikon Elements Analysis Stations

 

News:

New Computer installed on the N-STORM system

Additional Offline Image Analysis Workstation Available for use

Acknowledgement:

"(Microscopy) was performed at the University of Wisconsin-Madison Biochemistry Optical Core, which was established with support from the University of Wisconsin-Madison Department of Biochemistry Endowment"

 

Imaging techniques available include:

Standard wide-field fluorescence                     

Super- Resolution Microscopy (STORM/PALM; SIM)

Scanning Confocal

Fluorescence spectral detection

Live Cell Imaging

Single molecule imaging

TIRF

3D particle tracking

FRAP/FLIP

Deconvolution

Photoactivation/uncaging

Calcium imaging

FRET

Image Analysis Workstations

 

High resolution image of actin

N-SIM 3D reconstruction: dense core vesicles (green) actin (red) --- Image courtesy of Elle Grevstad

High resolution image of yfp

Confocal imaging of biofilms: Pseudomonas aeruginosa (yellow) Escherichia coli (cyan) --- Image courtesy of Katie Brenner (Weibel Lab)

High resolution image of c. elegans

N-SIM 3D reconstruction C.elegan gonad --- Image courtesy of Kim Haupt (Kimble Lab)

High resolution image of actin

N-SIM image of actin: depth coding --- Image courtesy of Elle Grevstad

High resolution image of e.coli biofilm

Confocal z-stack of e.coli biofilm

High resolution image of Mio

Confocal Spectral Imaging: Cos-1 cells, experimental cell tracker --- Image courtesy of Elle Grevstad