BIOCHEMISTRY

Collaborators

Stan Fields

University of Washington - Seattle

Functional genomics
We collaborate with Stan Fields' group to develop methods to analyze mRNA regulation on a genome-wide basis, including the three-hybrid system and genomic tethered function assays. 

Brian Kraemer, Beilin Zhang, Dhruba SenGupta, Stanley Fields and Marvin Wickens, 2000. A yeast three-hybrid system to detect and analyze interactions between RNA and protein. In RNA-Ligand Interactions. (Daniel W. Celander and John N. Abelson, eds.) of the Methods in Enzymology Series. Academic Press, New York. Vol. 328: Part III, pp.

Dhruba J. SenGupta, Marvin Wickens and Stanley Fields, 1999. Identification of RNAs that bind to a specific protein using the yeast three-hybrid system. RNA 5, 596-601. [PDF] [PubMed]

Dhruba SenGupta, Beilin Zhang, Brian Kraemer, Pascale Prochart, Stanley Fields and Marvin Wickens. 1996. A three-hybrid system for detecting RNA-protein interactions. Proc. Natl. Acad. Sci. 93, 8496-8501. [PDF] [PubMed]

Judith Kimble

University of Wisconsin - Madison

Regulatory networks in the C. elegans germline
Our collaboration with Judith Kimble's laboratory focuses on a network of mRNAs and proteins that together help coordinate development of a complex tissue, the germline.

Wang L, Kimble J, Wickens M. Tissue-specific modification of gld-2 mRNA in C. elegans: likely C-to-U editing. RNA. 2004 Sep;10(9):1444-8. [PDF] [PubMed]

Kwak JE, Wang L, Ballantyne S, Kimble J, Wickens M. Mammalian GLD-2 homologs are poly(A) polymerases. Proc Natl Acad Sci U S A. 2004 Mar 30;101(13):4407-12. [PDF] [PubMed]

Eckmann CR, Kraemer B, Wickens M, Kimble J. GLD-3, a bicaudal-C homolog that inhibits FBF to control germline sex determination in C. elegans. Dev Cell. 2002 Nov;3(5):697-710. [PDF] [PubMed]

Marvin Wickens, Elizabeth B. Goodwin, Judith Kimble, Sidney Strickland, and Matthias Hentze. Translational Control in Developmental Decisions. In Translational Control Second Edition. (Michael Mathews, ed.) Cold Spring Harbor Press, New York, Submitted.

B. Kraemer, S. Crittenden, M. Gallegos, G. Moulder. R. Barstead, J. Kimble and M. Wickens.. 1999. NANOS-3 and FBF proteins physically interact to control the sperm/oocyte switch in C. elegans. Current Biology 9, 1009-1018. [PDF] [PubMed]

A. Puoti, M. Gallegos, B. Zhang, M.P. Wickens and J. Kimble. 1997. Controls of cell fate and pattern by 3' untranslated regions: The Caenorhabditis elegans sperm/oocyte decision. Cold Spring Harbor Symposia on Quantitative Biology, 62, 19-24. [PubMed]

Beilin Zhang, Maria Gallegos, Alessandro Puoti, Eileen Durkin, Stanley Fields, Judith Kimble, and Marvin Wickens. 1997. A conserved RNA binding protein that regulates patterning of sexual fates in the C. elegans hermaphrodite germ line. Nature 390, 477-484. [PDF] [PubMed]

Jerry Yin

University of Wisconsin - Madison

Dr. Yin focuses on the molecular mechanisms of learning and memory, using Drosophila. We collaborate on aspects of mRNA control in the nervous system.

Traci Hall

NIH Research Triangle Park, NC

Our collaboration with Traci Hall focuses on the structural analysis of mRNA regulatory proteins.