Wickens Lab Logo

photo of Brad HookBrad Hook

Research Interests

Mechanisms of Eukaryotic Regulation
RNA-Protein Interactions
Mechanisms of Gene Regulation by RNA-binding Proteins that Interact with 3' Untranslated Regions

Education

University of Wisconsin-Madison (2000-Current)
Ph.D. in Biochemisty
Departments of Biochemistry

Indiana University (2000)
B.S. in Biochemistry
Honors in Biochemistry

Research

Graduate Research (2000-current)
Biochemical and Functional Characterization of PUF proteins
Principal Investigator: Dr. Marvin Wickens
Department of Biochemistry
University of Wisconsin-Madison

Undergraduate Research (1998-2000)
Using SELEX to find self-biotinylating RNAs
Principal Investigator: Dr. Donald Burke
Department of Chemistry
Indiana University

Teaching Experience

Instruction of undergraduate research projects
Principal Investigator: Dr. Marvin Wickens
Department of Biochemistry
University of Wisconsin-Madison

Teaching Assistant (Fall 2004 and Fall 2005)
Biochemistry 660, Biochemical Techniques
Instructor: Dr. Marvin Wickens
Department of Biochemistry
University of Wisconsin-Madison

Teaching Assistant (Spring 2002 and Fall 2002)
Biochemistry 501, Biochemistry
Instructor: Dr. Richard Amasino
Department of Biochemistry
University of Wisconsin-Madison

Teaching Assistant (Spring 2002 and Fall 2002)
Chemistry 341, Organic Chemistry Lecture
Instructor: Dr. Crandell
Department of Chemistry
Indiana University

Publications

Goldstrohm, A., B. Hook, et al. PUF protein control mRNAs by binding a deadenylase. Nat Struct Mol Biol In Review (2006).

Hook, B., Bernstein, D., Zhang, B. & Wickens, M. RNA-protein interactions in the yeast three-hybrid system: affinity, sensitivity, and enhanced library screening. RNA 11, 227-33 (2005).

Bernstein, D., Hook, B., Hajarnavis, A., Opperman, L. & Wickens, M. Binding specificity and mRNA targets of a C. elegans PUF protein, FBF-1. RNA 11, 447-58 (2005).

Opperman, L., Hook, B., Defino, M., Bernstein, D. S. & Wickens, M. A single spacer nucleotide determines the specificities of two mRNA regulatory proteins. Nat Struct Mol Biol (2005).

Lee, M. H., Hook, B., Lamont, L. B., Wickens, M. & Kimble, J. LIP-1 phosphatase controls the extent of germline proliferation in Caenorhabditis elegans. Embo J (2005).

Woycechowsky, K. J., Hook, B. A. & Raines, R. T. Catalysis of protein folding by an immobilized small-molecule dithiol. Biotechnol Prog 19, 1307-14 (2003).