BIOCHEMISTRY

Sindhu Chitteni Pattu - M.Sc., Ph.D                                                                         

Education:            

University of Wisconsin-Madison, USA (2002-2009) Post-doctoral

Sree Chitra Tirunal Institute for Medical Sciences and Technology, India (2001) Ph.D. Medical Microbiology

Mahatma Gandhi University, India (1995) M.Sc. Microbiology

University of Kerala, India (1993 B.Sc. Botany Major

Positions:                  

University of Wisconsin-Madison, USA (2013 – Present), Associate Researcher, Department of Biochemistry

University of Wisconsin-Madison, USA (2010 – 2013), Assistant Researcher, Department of Biochemistry

University of Wisconsin-Madison, USA (2002 – 2009), Research Associate, Institute for Molecular Virology and Department of Biochemistry

University of Wisconsin-Madison, USA (2000 – 2002), Research Specialist, Institute for Molecular Virology

Sree Chitra Tirunal Institute for Medical Sciences and Technology, India (1996-2000), Research Fellow, Department of Microbiology

Certifications:          

NIH Issued Certificate on IPPCR, Introduction to the principles and Practice of Clinical Research      

University of Wisconsin- Madison Issued Certificate on, Principles of Supervision and Management

Creating a Healthy, Inclusive, and Engaging Work Environment at the University of Wisconsin-Madison, Safety Fundamentals, Affirmative Action And Equal Employment Opportunity, Disability Accommodation, Workers' Compensation, Risk Management, Corrective Discipline Process, Family and Medical Leave Act (FMLA), Cybersecurity.

University of Wisconsin- Madison: Fully Prepared to Lead Certificate: (Ongoing)

Administrative Knowledge:              

UW- Madison Research Administration Basics

Expertise:                 

Transmission Electron Microscopy                                                                                                                  

RESEARCH:

Major Area:              Medical Microbiology, Biochemistry, Microscopy

Publications:            Peer-Reviewed Publications:

Leite, WC., Galvão, CW., Saab, SC., Iulek, J., Etto, RM., Steffens, MB., Chitteni-Pattu, S., Stanage, T., Keck, JL. and  Cox, MM. (2016) Structural and functional studies of H. seropedicae RecA Protein- Insights into the polymerization of RecA protein as nucleoprotein filament. PLoS One. Jul 22;11(7). PMID: 27447485

Kim, T., Chitteni-Pattu, S., Cox, BL., Wood, EA., Sandler, SJ. and Cox, MM. (2015) Directed evolution of RecA variants with enhanced capacity for conjugational recombination. PLoS Genet. Jun 5;11(6). PMID: 26047498

Petrova, V., Chen, S., Molzberger, E., Tomko, E., Chitteni-Pattu, S., Jia, H., Ordabayev, Y.,Lohman, T. and Cox, MM. (2015) Active displacement of RecA filaments by UvrD  translocase activity. Nucleic Acids Research.  Apr 30;43(8). PMID: 25824953

Piechura, JR., Tseng, TL., Hsu, HF., Byrne, RT., Windgassen, TA., Chitteni-Pattu, S.,  Battista, JR.,  Li, HW. and Cox, MM. (2015) Biochemical characterization of RecA variants that contribute to extreme resistance to ionizing radiation. DNA Repair (Amst). Feb (26): 30-43. PMID: 25559557 

Ngo, KV., Molzberger, ET., Chitteni-Pattu, S. and Cox, MM. (2013) Regulation of  Deinococcus radiodurans RecA protein function via modulation of active and inactive nucleoprotein filament states. Journal of Biological Chemistry. 288(29):21351-66. PMID: 23729671

George, NP., Ngo, KV., Chitteni-Pattu, S., Norais, CA., Battista, JR., Cox, MM. and Keck, JL. (2012) Structure and cellular dynamics of Deinococcus radiodurans single-stranded DNA (ssDNA)-binding protein (SSB)-DNA complexes. Journal of Biological Chemistry. 287(26):22123-32.  PMID:22570477

Hsu, HF., Ngo. KV., Chitteni-Pattu, S., Cox, MM. and  Li, HW. (2011) Investigating Deinococcus radiodurans RecA protein filament formation on double-stranded DNA by a real-time single-molecule approach. Biochemistry. 50(39):8270-80. PMID: 21853996

Britt, RL., Chitteni-Pattu, S., Page, AN. and Cox, MM. (2011) RecA K72R filament formation defects reveal an oligomeric RecA species involved in filament extension. Journal of Biological Chemistry. 286(10): 7830-40. PMID: 21193798

Gruenig, MC., Stohl, EA., Chitteni-Pattu, S., Seifert, HS. and Cox, MM. (2010) Less is more: Neisseria gonorrhoeae RecX protein stimulates recombination by inhibiting RecA. Journal of Biological Chemistry. 285(48): 37188-97. PMID: 20851893

Britt, RL., Haruta, N., Lusetti, SL.,  Chitteni-Pattu, S., Inman, RB. and Cox, MM. (2010) Disassembly of Escherichia coli RecA E38K/DeltaC17 nucleoprotein filaments is required to complete DNA strand exchange. Journal of Biological Chemistry. 285(5): 3211-26. PMID: 19910465

Petrova, V., Chitteni-Pattu, S., Drees, JC., Inman, RB. and Cox, MM. (2009) An SOS inhibitor that binds to free RecA protein: the PsiB protein. Molecular Cell .36(1):121-130. PMID: 19818715

Norais, CA., Chitteni-Pattu, S., Wood, EA., Inman, RB. and Cox, MM. (2009) DdrB protein, an alternative Deinococcus radiodurans SSB induced by ionizing radiation. Journal of Biological Chemistry 284(32): 21402-11. PMID: 19515845

Gruenig, MC., Renzette, N., Long, E., Chitteni-Pattu, S., Inman, RB., Cox, MM. and Sandler, SJ. (2008) Rec A-mediated SOS induction requires an extended filament conformation but no ATP hydrolysis. Molecular Microbiology 69(5): 1165-79. PMID: 18627467

Cox, JM., Li, H.,Wood, EA., Chitteni-Pattu, S., Inman, RB. and Cox, MM. (2008)  Defective dissociation of a "slow" RecA mutant protein imparts an Escherichia coli growth defect. Journal of Biological Chemistry 283(36):24909-21. PMID: 18603529

Cox, JM., Abbott, SN., Chitteni-Pattu, S., Inman, RB. and Cox, MM. (2006)    Complementation of one RecA protein point mutation by another. Evidence for Trans catalysis of ATP hydrolysis. Journal of Biological Chemistry 281(18): 12968-75. PMID: 16527806

Lusetti, SL., Hobbs, MD., Stohl, EA., Chitteni-Pattu, S., Inman, RB., Seifert, HS. and Cox, MM. (2006) The RecF protein antagonizes RecX function via direct interaction. Molecular Cell 21(1): 41-50. PMID: 16387652

Drees, JC., Chitteni-Pattu, S., McCaslin, DR., Inman, RB. and Cox, MM. (2006) Inhibition of RecA protein function by the RdgC protein from Escherichia coli. Journal of Biological Chemistry  281(8): 4708-17. PMID: 16377615

Drees, JC., Lusetti, SL., Chitteni-Pattu, S., Inman, RB. and Cox, MM. (2004) A RecA filament capping mechanism for RecX protein. Molecular Cell 15(5): 789-98. PMID: 15350222

Chitteni-Pattu, S. and Shanmugam, J. (1999) Correlation between slime production and multiple antibiotic resistance in Staphylococcus aureus. Biomedicine (19): 171-179. (Listed under Sindhu CP). IndMED: NI201885

Chitteni-Pattu, S. and Shanmugam, J. (1998) Beta-lactamase production

by Staphylococcus aureus and Coagulase Negative Staphylococci- detection by different iodometric and acidimetric techniques. Biomedicine (18): 74- 80. (Listed under Sindhu CP). IndMED: NI004135

Chitteni-Pattu, S., Shanmugam, J., Sankar Kumar, R., Antony, M., Mohansingh, MP. and Cherian, VK. (1997) Antibiogram pattern of Coagulase Negative Staphylococci isolated from cardiac surgery   patients during pre and post -operative periods. Biomedicine  (17): 63-70. (Listed under Sindhu CP). IndMED: NI004258