Vertebrate Animals

A separate attachment is required when you intend to use Vertebrate Animals wherein you are to address the following five key points.  There is no specific page limitation but you are instructed to be succinct.  NIH has developed an optional Vertebrate Animal Section Worksheet to help complete the Vertebrate Animals section.

  1. Provide a detailed description of the proposed use of animals in the work outlined in the Research Design and Methods section. Identify the species, strains, ages, sex, and numbers of animals to be used in the proposed work.
  2. Justify the use of animals, the choice of species, and the numbers to be used. If animals are in short supply, costly, or to be used in large numbers, provide an additional rationale for their selection and numbers.
  3. Provide information on the veterinary care of the animals involved.
  4. Describe the procedures for ensuring that discomfort, distress, pain, and injury will be limited to that which is unavoidable in the conduct of scientifically sound research. Describe the use of analgesic, anesthetic, and tranquilizing drugs and/or comfortable restraining devices, where appropriate, to minimize discomfort, distress, pain, and injury.
  5. Describe any method of euthanasia to be used and the reasons for its selection. State whether this method is consistent with the recommendations of the Panel on Euthanasia of the American Veterinary Medical Associate. If not, present a justification for not following the recommendations.