Robert N. Kirchdoerfer

Photo of Robert N. Kirchdoerfer
Assistant Professor (also Institute for Molecular Virology)
B.S., University of Wisconsin-Madison, Genetics and Biochemistry
Ph.D., The Scripps Research Institute
Postdoctoral, The Scripps Research Institute (Saphire laboratory)
Postdoctoral, The Scripps Research Institute (Ward laboratory)
Phone: (608) 262-6191
Email: rnkirchdoerf@wisc.edu

Publications

Jockusch S, Tao C, Li X, Anderson TK, Chien M, Kumar S, Russo JJ, Kirchdoerfer RN, Ju J. (2020) A Library of Nucleotide Analogues Terminate RNA Synthesis Catalyzed by Polymerases of Coronaviruses That Cause SARS and COVID-19. Antiviral Res. 2020 Jun 18;180:104857. doi: 10.1016/j.antiviral.2020.104857. Online ahead of print. PMID: 32562705; PMCID: PMC7299870. [PubMed]

Chien M, Anderson TK, Jockusch S, Tao C, Kumar S, Li X, Russo JJ, Kirchdoerfer RN, Ju J. (2020) Nucleotide Analogues as Inhibitors of SARS-CoV-2 Polymerase. bioRxiv. 2020 Mar 20:2020.03.18.997585. doi: 10.1101/2020.03.18.997585. Preprint. PMID: 32511320; PMCID: PMC7239050. [PubMed]

Kirchdoerfer RN. (2020) Halting Coronavirus Polymerase. J Biol Chem. 2020 Apr 10;295(15):4780-4781. doi: 10.1074/jbc.H120.013397. PMID: 32277065; PMCID: PMC7152748. [PubMed]

Nogal B, Bianchi M, Cottrell CA, Kirchdoerfer RN, Sewall LM, Turner HL, Zhao F, Sok D, Burton DR, Hangartner L, Ward AB. (2020) Mapping Polyclonal Antibody Responses in Non-human Primates Vaccinated With HIV Env Trimer Subunit Vaccines. Cell Rep. 2020 Mar 17;30(11):3755-3765.e7. doi: 10.1016/j.celrep.2020.02.061. PMID: 32187547;
PMCID: PMC7153566. [PubMed]

Lin AE, Diehl WE, Cai Y, Finch CL, Akusobi C, Kirchdoerfer RN, Bollinger L, Schaffner SF, Brown EA, Saphire EO, Andersen KG, Kuhn JH, Luban J, Sabeti PC. (2020) Reporter Assays for Ebola Virus Nucleoprotein Oligomerization, Virion-Like Particle Budding, and Minigenome Activity Reveal the Importance of Nucleoprotein Amino Acid Position 111. Viruses. 12(1) 2020 Jan 15. doi:10.3390/v12010105. PMID: 31952352. [PubMed]

Wang N, Rosen O, Wang L, Turner HL, Stevens LJ, Corbett KS, Bowman CA, Pallesen J, Shi W, Zhang Y, Leung K, Kirchdoerfer RN, Becker MM, Denison MR, Chappell JD, Ward AB, Graham BS, McLellan JS. (2019) Structural Definition of a Neutralization-Sensitive Epitope on the MERS-CoV S1-NTD. Cell Rep. Sep 24;28(13):3395-3405.e6.
doi: 10.1016/j.celrep.2019.08.052. PMID: 31553909; PMCID: PMC6935267. [PubMed]

Kirchdoerfer RN, Ward AB. (2019) Structure of the SARS-CoV nsp12 polymerase bound to nsp7 and nsp8 co-factors. Nat Commun. May 28;10(1):2342. doi: 10.1038/s41467-019-10280-3. PMID: 31138817; PMCID: PMC6538669 [PubMed]

Kirchdoerfer RN, Saphire EO, Ward AB.  (2019) Cryo-EM structure of the Ebola virus nucleoprotein-RNA complex. Acta Crystallogr F Struct Biol Commun. 2019 May 1;75(Pt 5):340-347. doi: 10.1107/S2053230X19004424. Epub 2019 Apr 24. PubMed PMID: 31045563; PubMed Central PMCID: PMC6497097. [PubMed]

Kirchdoerfer RN, Wang N, Pallesen J, Wrapp D, Turner HL, Cottrell CA, Corbett KS, Graham BS, McLellan JS, Ward AB. (2018) Stabilized coronavirus spikes are resistant to conformational changes induced by receptor recognition or proteolysis. Sci Rep. 2018 Oct 24;8(1):15701. doi: 10.1038/s41598-018-34171-7. Erratum in: Sci Rep. 2018 Dec 10;8(1):17823. PubMed PMID: 30356097; PubMed Central PMCID: PMC6200764. [PubMed]

King LB, Fusco ML, Flyak AI, Ilinykh PA, Huang K, Gunn B, Kirchdoerfer RN, Hastie KM, Sangha AK, Meiler J, Alter G, Bukreyev A, Crowe JE Jr, Saphire EO. (2018) The Marburgvirus-Neutralizing Human Monoclonal Antibody MR191 Targets a Conserved Site to Block Virus Receptor Binding. Cell Host Microbe. 2018 Jan 10;23(1):101-109.e4.
doi: 10.1016/j.chom.2017.12.003. PubMed PMID: 29324225; PubMed Central PMCID: PMC5772738. [PubMed]

Pallesen J, Wang N, Corbett KS, Wrapp D, Kirchdoerfer RN, Turner HL, Cottrell CA, Becker MM, Wang L, Shi W, Kong WP, Andres EL, Kettenbach AN, Denison MR, Chappell JD, Graham BS, Ward AB, McLellan JS. (2017) Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen. Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):E7348-E7357. doi: 10.1073/pnas.1707304114. Epub 2017 Aug 14. PubMed PMID: 28807998; PubMed Central PMCID: PMC5584442.

Kirchdoerfer RN, Wasserman H, Amarasinghe GK, Saphire EO. (2017) Filovirus Structural Biology: The Molecules in the Machine. Curr Top Microbiol Immunol. 2017;411:381-417. doi: 10.1007/82_2017_16. PubMed PMID: 28795188.

Bruhn JF, Kirchdoerfer RN, Urata SM, Li S, Tickle IJ, Bricogne G, Saphire EO. (2017) Crystal Structure of the Marburg Virus VP35 Oligomerization Domain. J Virol. 2017 Jan 3;91(2). pii: e01085-16. doi: 10.1128/JVI.01085-16. Print 2017 Jan 15. PubMed PMID: 27847355; PubMed Central PMCID: PMC5215338.

Kirchdoerfer RN, Moyer CL, Abelson DM, Saphire EO. (2016) The Ebola Virus VP30-NP Interaction Is a Regulator of Viral RNA Synthesis. PLoS Pathog. 2016 Oct 18;12(10):e1005937. doi: 10.1371/journal.ppat.1005937. eCollection 2016 Oct. PubMed PMID: 27755595; PubMed Central PMCID: PMC5068707.

Kirchdoerfer RN, Cottrell CA, Wang N, Pallesen J, Yassine HM, Turner HL, Corbett KS, Graham BS, McLellan JS, Ward AB. (2016) Pre-fusion structure of a human coronavirus spike protein. Nature. 2016 Mar 3;531(7592):118-21.
doi: 10.1038/nature17200. PubMed PMID: 26935699; PubMed Central PMCID: PMC4860016.

Kirchdoerfer RN, Abelson DM, Li S, Wood MR, Saphire EO. (2015) Assembly of the Ebola Virus Nucleoprotein from a Chaperoned VP35 Complex. Cell Rep. 2015 Jul 7;12(1):140-149. doi: 10.1016/j.celrep.2015.06.003. Epub 2015 Jun 25. PubMed PMID: 26119732; PubMed Central PMCID: PMC4500542.

Clifton MC, Kirchdoerfer RN, Atkins K, Abendroth J, Raymond A, Grice R, Barnes S, Moen S, Lorimer D, Edwards TE, Myler PJ, Saphire EO. (2014) Structure of the Reston ebolavirus VP30 C-terminal domain. Acta Crystallogr F Struct Biol Commun. 2014 Apr;70(Pt 4):457-60. doi: 10.1107/S2053230X14003811. Epub 2014 Mar 25. PubMed PMID: 24699737; PubMed Central PMCID: PMC3976061.

Lee JY, Kim HS, Baek IW, Back JM, Han MR, Kong SY, Kim JH, Kirchdoerfer RN, Kim JO, Cooper MD, Wilson IA, Kim HJ, Han BW. (2013) Overexpression, crystallization and preliminary X-ray crystallographic analysis of the variable lymphocyte receptor 2913 ectodomain fused with internalin B. Acta Crystallogr Sect F Struct Biol Cryst Commun.
2013 Jan 1;69(Pt 1):39-41. doi: 10.1107/S1744309112045484. Epub 2012 Dec 20. PubMed PMID: 23295483; PubMed Central PMCID: PMC3539700.

Moeller A, Kirchdoerfer RN, Potter CS, Carragher B, Wilson IA. (2012) Organization of the influenza virus replication machinery. Science. 2012 Dec 21;338(6114):1631-4. doi: 10.1126/science.1227270. Epub 2012 Nov 22. PubMed
PMID: 23180774; PubMed Central PMCID: PMC3578580.

Kirchdoerfer RN, Herrin BR, Han BW, Turnbough CL Jr, Cooper MD, Wilson IA. (2012) Variable lymphocyte receptor recognition of the immunodominant glycoprotein of Bacillus anthracis spores. Structure. 2012 Mar 7;20(3):479-86.
doi: 10.1016/j.str.2012.01.009. PubMed PMID: 22405006; PubMed Central PMCID: PMC3337218.

Kirchdoerfer RN, Garner AL, Flack CE, Mee JM, Horswill AR, Janda KD, Kaufmann GF, Wilson IA. (2011) Structural basis for ligand recognition and discrimination of a quorum-quenching antibody. J Biol Chem. 2011 May 13;286(19):17351-8.
doi: 10.1074/jbc.M111.231258. Epub 2011 Mar 23. PubMed PMID: 21454495; PubMed Central PMCID: PMC3089576.

Ellis JP, Bakke CK, Kirchdoerfer RN, Jungbauer LM, Cavagnero S. (2008) Chain dynamics of nascent polypeptides emerging from the ribosome. ACS Chem Biol. 2008 Sep 19;3(9):555-66. doi: 10.1021/cb800059u. Epub 2008 Aug 22. PubMed PMID:18717565; PubMed Central PMCID: PMC2572860.

Debler EW, Kaufmann GF, Kirchdoerfer RN, Mee JM, Janda KD, Wilson IA. (2007) Crystal structures of a quorum-quenching antibody. J Mol Biol. 2007 May 18;368(5):1392-402. Epub 2007 Mar 6. PubMed PMID: 17400249; PubMed Central
PMCID: PMC1994716.

Kirchdoerfer RN, Huang JJ, Isola MK, Cavagnero S. (2007) Fluorescence-based analysis of aminoacyl- and peptidyl-tRNA by low-pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal Biochem. 2007 May 1;364(1):92-4. Epub 2007 Jan 19. PubMed PMID: 17368419; PubMed Central PMCID: PMC2693959.