BIOCHEMISTRY

Key Steps to Generating High Quality Real-Time PCR (RT-qPCR) Data that Meets the MIQE Guidelines

In an effort to assist the scientific community to produce consistent, high quality data from RT-qPCR experiments, The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines were recently published (Bustin et al. 2009). The ultimate goal of MIQE is to establish clear guidelines for the information required to publish RT-qPCR data to allow reviewers and editors to measure the technical quality of submitted manuscripts against an established yardstick and to facilitate easier replication of experiments described in published studies. This talk focuses on how to apply the MIQE guidelines to design a solid experimental
approach for RT-qPCR.