Role of DNA Structural Variability in Promoter Function
Molecular Biophysics Unit
Indian Institute of Science
The talk will outline some of our recent studies wherein structural variability or flexibility in DNA is found to be strongly correlated with its function.
Recent experimental and in silico analyses of promoters indicate that while there is considerable variation in their base composition and sequence, leading to presence of some non-B-DNA structural motifs, some DNA structural properties also show significant conservation. We have analyzed various structural features, such as stability, bendability (using both DNase1 sensitivity and nucleosome positioning preference models), intrinsic curvature and groove width, in promoter regions of prokaryotic and eukaryotic genomes and compared these with coding regions. Published experimental data from genome wide transcriptome analysis of various prokaryotes, correlates well with DNA structural features and their variability in different classes of promoters. It is also observed that structural features of sequences flanking the consensus binding sites of transcription factors are strongly correlated with their binding affinity.