BIOCHEMISTRY

The BD LSRFortessa is an air-cooled multi-laser benchtop flow cytometer with the ability to acquire parameters for a large number of colors. It uses fixed-alignment lasers that transmit light through a flow cell to configurable octagon and trigon detector arrays. These detectors collect and translate the resulting fluorescence signals into electronic signals. Cytometer electronics convert these signals into digital data.

Determine the mass of a biological molecule.

A chemostat allows us to study interactions between members of microbial communities for a prolonged period of time where pH, nutrient availability and washout of waste products/dead cells can be precisely controlled. This system will be used for many projects in the lab including studying the metabolism of synthetic human gut microbiome communities, response to invasion by intestinal pathogens, control of microbial communities using engineered probiotics and phage-bacterial interactions. In addition, a major challenge for interpreting ‘omics’ data in batch culture is that the environment and thus the cellular physiological state is continuously changing. Therefore, the continuous culture system would establish precise control of the environment for investigation of global mRNA, protein and metabolite profile

SPR optical biosensor that provides real-time data on the affinity, specificity, and interaction kinetics of protein interactions.