Biochem Equipment | Page 3

Biochemistry Departmental Equipment

The equipment listed on this page is typically available for all users in the department to access for their research needs. By sharing the equipment labs are able to access equipment that is either not regularly used or might be cost prohibitive for an individual lab to purchase and maintain.

Keyence BZ-X800E

Function:

Examine intracellular RNA virus replication and the formation of viral replicative compartments. In addition, the ability of this microscope to perform imaging on live cells in a plate-based format best enables the optimization of dual fluorescence viral replicon assays using image cytometry

Looking Glass holographic display

Function:

This display can project images in 3D but no VR goggles are needed. This display would best be used for allowing teams or small class groups to visualize a structure in 3D simultaneously. While it is not as immersive as a VR experience, it would facilitate collaborative interactions with larger groups simultaneously. This could potentially be installed in one permanent location (BIF/BOC, cryo-EM facility, Or even room 2317) or mounted on a stand so that it can be “checked out” by users

Low-Volume Nano ITC

Function:

Measure molecular binding events in small volumes.

LSRFortessa X20 Standard

Function:

The BD LSRFortessa is an air-cooled multi-laser benchtop flow cytometer with the ability to acquire parameters for a large number of colors. It uses fixed-alignment lasers that transmit light through a flow cell to configurable octagon and trigon detector arrays. These detectors collect and translate the resulting fluorescence signals into electronic signals. Cytometer electronics convert these signals into digital data.

MakerBot 3D Printer

Function:

3D printer for creating prototypes using PLA filaments

MALDI TOF-TOF Mass Spectrometer

Function:

Determine the mass of a biological molecule.

MetaMorph Software Suite

Function:

Analysis of images and movies.

Microfluidics microfluidizer

Function:

This instrument is adequate for processing relatively fragile Gram-negative organisms such as E. coli.

Multifors II Chemostat

Function:

A chemostat allows us to study interactions between members of microbial communities for a prolonged period of time where pH, nutrient availability and washout of waste products/dead cells can be precisely controlled. This system will be used for many projects in the lab including studying the metabolism of synthetic human gut microbiome communities, response to invasion by intestinal pathogens, control of microbial communities using engineered probiotics and phage-bacterial interactions. In addition, a major challenge for interpreting ‘omics’ data in batch culture is that the environment and thus the cellular physiological state is continuously changing. Therefore, the continuous culture system would establish precise control of the environment for investigation of global mRNA, protein and metabolite profile