Titan Krios G3i with FFI and micro-ED upgrades

Function: 
The Krios microscope is used primarily for single particle cryo-EM and cryo-electron tomography (cryo-ET) data collection on a wide-range of investigator-delivered biological, (bio)chemical, and materials science samples
Contact Info: 

Elizabeth R. Wright

E-mail erwright2@wisc.edu

Phone (608) 265-0666

Oversight Plan: 

The FFI and micro-ED equipment will be installed on the existing Titan Krios G3i cryo-TEM in the UWMadison,
Department of Biochemistry Cryo-EM Research Center. The instruments in the Cryo-EM Research
Center are overseen and maintained by well-trained staff, ThermoFisher field service engineers, and
maintenance contracts with ThermoFisher. Instrument access and training in the Cryo-EM Research Center is
managed via iLab bookings. From an initial booking, Cryo-EM Research Center staff coordinate with the
applicant (user) to schedule instrument access and training. Since the inception of the Cryo-EM Research
Center’s user access and training program in mid-2021, the facility has supported 49 users from 30 research
labs (27 users from 13 Department of Biochemistry labs). We envision that with the consistent growth of single
particle cryo-EM and micro-ED amongst investigators in the Department of Biochemistry and across UWMadison,
that these numbers will increase significantly

Training Plan: 

The Cryo-EM Research Center develops and provides all facility users with easy to follow instructional
materials for all instruments housed in the Center. The Cryo-EM Research Center staff review materials and
train regularly with the ThermoFisher applications team to keep abreast with new technologies, approaches, or
regular instrument updates that are then adapted to center operations and training protocols. Upon an
investigator booking in iLab, the management team reviews the request, schedules a meeting, and determines
the needs of the individual user. As a user onboards with the Cryo-EM Research Center, they are paired with a
facility member for one-on-one training on preparative equipment and microscopes. Once a trainee has
received training, practiced on an instrument, and ‘checked out’ with their trainer, they are designated as an
independent user and may book equipment on an as needed basis. If a user has not worked with equipment
for an extended period, they may schedule refresher training with Cryo-EM Research Center staff members.